Granulated and Non-Granulated Decidual Prolactin-Related Protein-Positive Decidual Cells in the Pregnant Mouse Endometrium

Authors

  • Luciane Candeloro,

    1. Laboratory of Biology of Reproduction and Extracellular Matrix, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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  • Telma M.T. Zorn

    1. Laboratory of Biology of Reproduction and Extracellular Matrix, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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Telma M.T. Zorn, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, Av. Lineu Prestes, 1524, CEP 05508-900, São Paulo, Brazil.
E-mail: temtzorn@usp.br

Abstract

Problem

Identification of the cell types responsible for the synthesis of decidual prolactin-related protein (dPRP) in the pregnant mouse endometrium.

Method of study

Histochemistry and immunocytochemistry were used to determine peri-implantation dPRP and perlecan distribution in the mouse uterus.

Results

We identified dPRP in pre-decidual and mature decidual cells from days 5 to 12 of pregnancy. On day 8, dPRP immunoreactivity was detected within cytoplasmic granules of a specific population of granulated decidual cells (GDCs). In mesometrial decidual cells, weak immunoreactivity was seen from days 7 to 14. Between days 11 and 14, dPRP was found in cytoplasm and in the extracellular matrix surrounding islands of spongiotrophoblast. Perlecan, a heparan sulfate proteoglycan, was co-localized with dPRP.

Conclusion

GDCs are a putative source of dPRP in pregnant mice. Co-localization of perlecan with dPRP suggests that the former acts as a dPRP reservoir and facilitates its paracrine effect in developing placental tissues.

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