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Tumor Necrosis Factor-alpha in Gestation and Puerperium of Women with Gestational Hypertension and Pre-eclampsia

Authors

  • José Carlos Peraçoli,

    1. Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, Botucatu, São Paulo, Brazil;
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  • Marilza Vieira Cunha Rudge,

    1. Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, Botucatu, São Paulo, Brazil;
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  • Maria Terezinha Serrão Peraçoli

    1. Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University, Botucatu, São Paulo, Brazil
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José Carlos Peraçoli, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970 Botucatu, São Paulo, Brazil.
E-mail: peracoli@fmb.unesp.br

Abstract

Problem

High plasma levels of tumor necrosis factor-alpha (TNF-α) in pregnant women have been associated with the pathogenesis of pre-eclampsia (PE). This study evaluated TNF-α plasma levels and monocyte production in gestational hypertension (GH) and PE during gestation and at puerperium.

Method of study

This study included 128 women, of whom 20 were non-pregnant (NP) normotensive (NT), and 108 were pregnant: 36 NT, 27 with GH, and 45 with PE. Peripheral blood plasma was used for TNF-α and uric acid determination. TNF-α was determined in plasma and lipopolysaccharide (LPS)-stimulated and non-stimulated monocyte supernatants by L929 bioassay.

Results

Tumor necrosis factor-alpha and uric acid plasma levels were higher in PE than in GH pregnancies. In both hypertensive groups, these parameters positively correlated and were significantly more elevated than in NT and NP women. TNF-α plasma levels and monocyte production were higher in hypertensive than in NT women during gestation, and significantly decreased at puerperium. Although decreased, TNF-α release in LPS-stimulated PE monocytes, was still significantly higher than in the other pregnant groups.

Conclusion

In vivo monocyte activation in GH and PE pregnant women was characterized by in vitro TNF-α production. The fact that higher circulating concentrations of TNF-α and uric acid were observed in PE than in GH suggests an association with disease severity.

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