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JEG-3 Cell Culture Supernatants Cause Reduced Interferon-γ and Interleukin-17 Production in Mixed-Lymphocyte Reactions

Authors


Dr. Sutatip Pongcharoen, Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand 65000.
E-mail: sutatipp@nu.ac.th

Abstract

Problem

Immunoregulatory effects of choriocarcinoma-derived factors on leukocytes have been documented. The present study was designed to investigate the effect of JEG-3 culture supernatants on interferon-γ (IFN-γ), interleukin-17 (IL-17) and IL-1β production in the mixed lymphocyte reactions (MLRs).

Method of study

A human choriocarcinoma cell line JEG-3 was used to test the effects of its culture supernatants on the proliferation and cytokine production in the MLRs. The cell proliferation was assessed using the BrdU incorporation and the amounts of cytokines were measured using enzyme-linked immunosorbent assays.

Results

The JEG-3 culture supernatants caused significantly reduced IFN-γ and IL-17 production in the MLRs. However, the supernatants did not influence MLR production of IL-1β.

Conclusion

IFN-γ and IL-17 are mainly produced by activated T cells but IL-1β is primarily produced by monocytes, thus suggesting that immunoregulatory factors of JEG-3 cells selectively inhibit cytokine production by activated T cells rather than that of the monocytes.

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