Distribution and Spatiotemporal Relationship of Activin A and Follistatin in Mouse Decidual and Placental Tissue

Authors

  • L. Candeloro,

    1. Laboratory of Rebroductive and Extracelluar Matrix Biology, Department of Cell and Development Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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  • Telma M. T. Zorn

    1. Laboratory of Rebroductive and Extracelluar Matrix Biology, Department of Cell and Development Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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Telma M. T. Zorn, Laboratory of Reproductive and Extracelluar Matrix Biology, Department of Cell and Development Biology, Institute of Biomedical Sciences, University of São Paulo, Av. Dr Lineu Prestes 1524, CEP: 05508-900, São Paulo, SP, Brazil.
E-mail: temtzorn@usp.br

Abstract

Problem

Cells responsible for the synthesis of follistatin and activin A in the pregnant mouse endometrium have not been characterized.

Method of study

Immunocytochemistry was used to determine the distribution of follistatin and activin A in the pregnant mouse uterus.

Results

Follistatin was detected in the endometrium prior to decidualization and embryo implantation. Follistatin was not seen in fully decidualized cells, being restricted to non-decidualized fibroblasts and cells in the process of decidualization. In contrast, activin A was detected exclusively in mature antimesometrial decidual cells during involution. After day eleven of pregnancy, both substances were identified in the extracellular matrix of the spongiotrophoblast.

Conclusion

As previously described for decidual prolactin-related protein and the proteoglycan perlecan, follistatin and activin A were detected in the extracellular matrix of the spongiotrophoblast, suggesting that this region acts as reservoir for these growth factors in the mouse placenta.

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