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Keywords:

  • Antibody response;
  • B-cell activation;
  • IL-13;
  • immunoglobulin;
  • sexual dichotomy

Problem  Dalton’s lymphoma (DL) shows very high interleukin (IL)-13 level in the serum and ascitic fluid. Therefore, in the present study, we investigated if any sexual dichotomy in the level of IL-13, and resulting B-cell activation and Ig subclass switching also exist in the tumor-bearing host.

Method of study  Serum and ascitic fluid of different groups of DL-bearing mice were isolated and IL-13 level and various Ig levels in serum were quantified by double-sandwich enzyme-linked immunosorbent assay. Further, the B cells were isolated from DL-bearing mice and effects of various concentrations of IL-13 and sex steroids were measured.

Results  Uncastrated and hormone replaced DL-bearing mice showed sexual dichotomy in IL-13 level, and subsequent difference in Ig-level and this was found to be more pronounced in females. Similarly, in vitro study suggested that estrogen treatment, in combination with IL-13, strongly modulates B-cell Ig switching in comparison to testosterone treatment in association with IL-13.

Conclusion  It can be concluded that IL-13, in concert with gonadal hormones, differentially modulates the B-cell function in a tumor-bearing host.