ORIGINAL ARTICLE: Gender Dichotomy in Antibody Response in a T-Cell Lymphoma: Involvement of IL-13 and Gonadal Hormones
Article first published online: 24 OCT 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
American Journal of Reproductive Immunology
Volume 59, Issue 2, pages 127–138, February 2008
How to Cite
Deepak, P., Kumar, S. and Acharya, A. (2008), ORIGINAL ARTICLE: Gender Dichotomy in Antibody Response in a T-Cell Lymphoma: Involvement of IL-13 and Gonadal Hormones. American Journal of Reproductive Immunology, 59: 127–138. doi: 10.1111/j.1600-0897.2007.00541.x
- Issue published online: 24 OCT 2008
- Article first published online: 24 OCT 2008
- Submitted May 8, 2007; accepted September 13, 2007.
- Antibody response;
- B-cell activation;
- sexual dichotomy
Problem Dalton’s lymphoma (DL) shows very high interleukin (IL)-13 level in the serum and ascitic fluid. Therefore, in the present study, we investigated if any sexual dichotomy in the level of IL-13, and resulting B-cell activation and Ig subclass switching also exist in the tumor-bearing host.
Method of study Serum and ascitic fluid of different groups of DL-bearing mice were isolated and IL-13 level and various Ig levels in serum were quantified by double-sandwich enzyme-linked immunosorbent assay. Further, the B cells were isolated from DL-bearing mice and effects of various concentrations of IL-13 and sex steroids were measured.
Results Uncastrated and hormone replaced DL-bearing mice showed sexual dichotomy in IL-13 level, and subsequent difference in Ig-level and this was found to be more pronounced in females. Similarly, in vitro study suggested that estrogen treatment, in combination with IL-13, strongly modulates B-cell Ig switching in comparison to testosterone treatment in association with IL-13.
Conclusion It can be concluded that IL-13, in concert with gonadal hormones, differentially modulates the B-cell function in a tumor-bearing host.