Michele Milanese and Ludovica Segat equally contributed to this work.
ORIGINAL ARTICLE: MBL2 Genetic Screening in Patients with Recurrent Vaginal Infections
Article first published online: 24 OCT 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
American Journal of Reproductive Immunology
Volume 59, Issue 2, pages 146–151, February 2008
How to Cite
Milanese, M., Segat, L., De Seta, F., Pirulli, D., Fabris, A., Morgutti, M. and Crovella, S. (2008), ORIGINAL ARTICLE: MBL2 Genetic Screening in Patients with Recurrent Vaginal Infections. American Journal of Reproductive Immunology, 59: 146–151. doi: 10.1111/j.1600-0897.2007.00549.x
- Issue published online: 24 OCT 2008
- Article first published online: 24 OCT 2008
- Submitted July 26, 2007; accepted October 12, 2007.
- bacterial vaginosis;
- innate immunity;
- melting temperature assay;
- vulvovaginal candidiasis
Problem Mannose-binding lectin (MBL) is an important component of the innate immunity, present at the mucosal level in vagina: a common pathogen’s entry point.
Method of study We used a rapid genotyping method based on melting temperature assay to search for three single nucleotide polymorphisms (SNPs) located in the first exon of the MBL2 gene and we also measured MBL serum levels in patients with recurrent bacterial vaginosis (rBV) and recurrent vulvovaginal candidiasis (rVVC).
Results Detected frequencies of MBL2 SNPs were comparable to the ones already reported for the Italian population and no significant differences were found between rVVC, rBV and controls. MBL serum levels did not show significant differences between the studied groups.
Conclusion No correlation for the screened mutations has been found neither in protecting nor in favoring the infection in rVVC and rBV patients. Our data demonstrate a lack of association between functional polymorphisms in the first exon of MBL2 gene, MBL deficiency,VVC and rBV.