ORIGINAL ARTICLE: Lack of Consistent Association Between Endothelial Nitric Oxide Synthase Gene Polymorphisms, Homocysteine Levels and Recurrent Pregnancy Loss in Tunisian Women

Authors

  • Walid Zammiti,

    1. Research unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, Monastir University, Monastir, Tunisia
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  • Nabil Mtiraoui,

    1. Research unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, Monastir University, Monastir, Tunisia
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  • Touhami Mahjoub

    1. Research unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, Monastir University, Monastir, Tunisia
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Walid Zammiti, Faculté de Pharmacie, Rue Ibn sina, Monastir, CP 5000, Tunisia. E-mail: zammiti_pharmacie@yahoo.fr

Abstract

Problem  Polymorphisms of the endothelial nitric oxide synthase (eNOS) gene have been associated with reduced vascular NO production or increased level of homocysteine, and evaluated as risk factors for recurrent pregnancy loss (RPL). Therefore, in this case-control study, we aimed to determine the effects of some eNOS functional polymorphisms: the 27-bp intron 4 repeat, the 894G/T of exon 7, and the promoter substitution -786T/C, in women with RPL.

Method of study  We genotyped 350 patients with RPL and 200 healthy women by polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR). The homocysteine total plasma concentrations (tHcy) were determined by enzyme-linked immunosorbent assay (ELISA).

Results  None of the eNOS polymorphisms-related alleles, genotypes, and haplotypes were associated with RPL. The tHcy were similar between patients and controls; no significant association between tHcy levels and eNOS genotypes could be evidenced.

Conclusion  The present study identified a lack of association between eNOS gene polymorphisms, the risk of RPL and tHcy levels.

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