ORIGINAL ARTICLE: Th1 and Th2 in Human IVF Pregnancy with Allogenic Fetus
Article first published online: 11 MAR 2008
© 2008 The Authors
American Journal of Reproductive Immunology
Volume 59, Issue 4, pages 352–358, April 2008
How to Cite
Chernyshov, V. P., Tumanova, L. E., Sudoma, I. A. and Bannikov, V. I. (2008), ORIGINAL ARTICLE: Th1 and Th2 in Human IVF Pregnancy with Allogenic Fetus. American Journal of Reproductive Immunology, 59: 352–358. doi: 10.1111/j.1600-0897.2007.00578.x
- Issue published online: 11 MAR 2008
- Article first published online: 11 MAR 2008
- Submitted October 20, 2007; accepted December 19, 2007.
- Chemokine receptors;
- oocyte donation;
Problem Positive obstetric outcome of allogenic in vitro fertilization (IVF) pregnancies makes interesting the subject of additional regulatory mechanisms after oocyte donation.
Method of study Eighty eight women: 23 donation of oocytes (DO), 33 IVF, 32 natural conception (NC) were studied in first trimester of pregnancy. Intracellular production of cytokines and chemokine receptors expression were studied by flow cytometry.
Results Intracellular production of interferon-γ (IFN-γ), interleukin (IL)-4, tumor necrosis factor-alpha by CD4 T lymphocytes in DO women was higher than in IVF and NC women. Ratio IFN-γ/IL-4 in DO was lower than in IVF. We found higher expression of chemokine receptor CCR4 but not CXCR3 on CD4 T cells in DO compared with IVF and NC. Ratio CXCR3/CCR4 in DO was lower than in NC.
Conclusion Hyperactivation of T helper 1 (Th1) and T helper 2 (Th2) by allogenic fetus is specific for DO pregnancy. Preferable activation of Th2 and relative suppression of Th1 chemokine expression reflect additional regulatory counteractive mechanism(s).