ORIGINAL ARTICLE: Immunophenotype of Peripheral T Lymphocytes, NK Cells and Expression of CD69 Activation Marker in Patients with Recurrent Spontaneous Abortions, During the Mid-Luteal Phase
Version of Record online: 28 JUN 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
American Journal of Reproductive Immunology
Volume 60, Issue 1, pages 66–74, July 2008
How to Cite
Prado-Drayer, A., Teppa, J., Sánchez, P. and Camejo, M. I. (2008), ORIGINAL ARTICLE: Immunophenotype of Peripheral T Lymphocytes, NK Cells and Expression of CD69 Activation Marker in Patients with Recurrent Spontaneous Abortions, During the Mid-Luteal Phase. American Journal of Reproductive Immunology, 60: 66–74. doi: 10.1111/j.1600-0897.2008.00591.x
- Issue online: 28 JUN 2008
- Version of Record online: 28 JUN 2008
- Submitted December 8, 2007; accepted February 4, 2008.
- natural killer cells;
- recurrent spontaneous abortion;
- T lymphocytes
Problem Determination of subpopulations of T lymphocytes, natural killer (NK) and activation status, in peripheral blood during the mid-luteal phase from patients with unexplained recurrent spontaneous abortion (RSA).
Method of study Peripheral blood samples from non-pregnant women with RSA and normal multiparous were taken and evaluated for subpopulations of T lymphocytes: CD4, CD8, (‘naive-like’ and ‘memory-like’), TCR receptor (αβ and γδ), activation status by CD69+surface or intracellular/CD3+, and NK cells (CD16+/CD56dim/CD3−, CD16+/CD56 bright/CD3−, CD69+surface or intracellular/CD56+/CD3− cells).
Results The evaluation of T lymphocytes only showed an increase in the expression of CD69 (surface and intracellular) in the RSA group. Additionally, we observed an increase in the total NK cells, CD56+ NK cells percentages, CD56dim NK cells and CD69 NK cells in RSA group.
Conclusion These observations support the concept that immunological activation of T lymphocytes and NK cells could be involved in peripheral blood during the mid-luteal phase in patients with unexplained RSA.