ORIGINAL ARTICLE: Decidual Natural Killer Cell Tuning by Autologous Dendritic Cells
Article first published online: 9 APR 2008
© 2008 The Authors
American Journal of Reproductive Immunology
Volume 59, Issue 5, pages 433–445, May 2008
How to Cite
Laškarin, G., Redžović, A., Rubeša, Ž., Mantovani, A., Allavena, P., Haller, H., Vlastelić, I. and Rukavina, D. (2008), ORIGINAL ARTICLE: Decidual Natural Killer Cell Tuning by Autologous Dendritic Cells. American Journal of Reproductive Immunology, 59: 433–445. doi: 10.1111/j.1600-0897.2008.00599.x
- Issue published online: 9 APR 2008
- Article first published online: 9 APR 2008
- Submitted January 11, 2008; accepted February 11, 2008.
- cytolytic mediators;
- DC/NK interaction;
- NK proliferation;
Problem Dendritic cells (DC)/natural killer (NK) cells interactions in the deciduas of early human pregnancies were analyzed in vitro.
Method of study Phenotype, cytokine expression and/or cytolytic mediators’ expression were measured by flow cytometry in NK and DC from the freshly isolated decidual mononuclear cells or after their purification and co-culture in vitro. Proliferation of 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE)-labeled CD56+ cells was analyzed by flow cytometry after the co-culture with CD1a+ or CD83+ DC.
Results Decidual CD1a+ cells show less mature phenotype with no expression of CD197, lower expression of CD80 and CD86 and higher expression of CD206 and CD195 in comparison to CD83+ cells. Interleukin (IL)-15, interferon-γ and tumor necrosis factor-α productions were higher in immature than mature DC, whereas IL-10 and IL-18 were equally produced in both subpopulations. Immature DC increase perforin, FasL and TRAIL protein expression and proliferation of NK cells, but decrease their intracellular IL-15 production. Mature DC caused less efficient proliferation of NK cells, and did not affect cytokine and cytolytic mediator expression.
Conclusion These results suggest that decidual CD1a+ cells regulate and shape NK cell function more profoundly than CD83+ cells in decidua.