ORIGINAL ARTICLE: High Expression of l-Selectin Ligand in Secretory Endometrium is Associated with Better Endometrial Receptivity and Facilitates Embryo Implantation in Human Being

Authors


Huang He-Feng, Department of Reproductive Endocrinology, Women’s Hospital, School of Medicine, Zhejiang University, 2 Xue Shi Road, Hangzhou, Zhejiang, 310006, China.
E-mail: huanghefg@hotmail.com

Abstract

Problem l-selectin ligand has displayed mediating adhesion at the maternal–fetal interface. Therefore, we investigated the impact of l-selectin ligand on establishing pregnancy in women undergoing in vitro fertilization and embryo transfer (IVF-ET).

Method of study  Endometrium between cycle days LH +6 to +9 was obtained from 56 Chinese women referred for IVF and tested for l-selectin ligand by immunohistochemistry and Western blot. The standard gonadotropin-releasing hormone agonist long protocol was used for ovarian stimulation.

Results l-selectin ligand was localized in the endometrial gland and luminal epithelial cells. Western blot analysis of endometrium identified four bands and levels of component 1, 2 and 4 were significantly higher in the pregnancy group than in the non-pregnancy group (< 0.05). Clinical pregnancy and implantation rates were higher in patients with high level l-selectin ligand compared with those with low level (53.6%versus 25.0%, and 27.1%versus 12.1%, respectively, < 0.05).

Conclusion  The presence of higher level l-selectin ligand was associated with a better pregnancy outcome.

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