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ORIGINAL ARTICLE: Association of Tumor Necrosis Factor-Alpha and Interleukin-10 Gene Polymorphisms in Iranian Patients with Pre-eclampsia


Mahroo Mirahmadian, PhD, Department of Immunology, School of Medicine, Medical Sciences/University of Tehran, PO Box 14155-3676, Tehran, Iran.


Problem  Considering that certain cytokines may change during pre-eclampsia (PE), because of functional polymorphisms in their genes, our purpose was to determine the association between tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) gene polymorphisms and development of PE.

Method of study  The genetic polymorphisms of TNF-α and IL-10 was studied by polymerase chain reaction-sequence specific primers in the DNA of peripheral blood cell from 160 patients with PE and 100 healthy pregnant women.

Results  We found a significant difference between TNF-α A allele (−308) and G allele (−238) in PE patients compared with those of the control groups. A significantly higher C/C genotype frequency of IL-10 (−592 and −819) was observed in the PE patients than in the control groups. In addition, the frequencies of three common IL-10 haplotypes (GCC, ACC, and ATA) did not show any significant difference between the study groups.

Conclusion  These findings would support the concept of contribution of TNF-α and IL-10 gene polymorphisms in the pathogenesis of PE in our population.