ABSTRACTS: 3
Development of an in vitro model for human embryo implantation and progesterone regulation

4TH EMBIC SUMMER SCHOOL, BARCELONA, SPAIN, 2TH -6TH JUNE 2008 TOP SELECTED ABSTRACTS

Authors


Abstract

Aim:  Develop an in vitro 3D-model for human embryo implantation on receptive endometrial construct.

Material and Methods:  Three-dimensional in vitro endometrial construct (n=12) was developed by culturing isolated human endometrial stromal cells in collagen matrix overlaid with epithelial cells. After priming hormonally, human blastocysts were placed and cultured in alpha-medium. Attachment of embryo was confirmed using cytokeratin-7 and expression of endometrial receptivity markers using immunohistochemistry. Antiprogestin mifepristone was used to test implantation and receptivity.

Results:  Embryos attached to receptive endometrial construct. Epithelial and stromal cells of endometrial construct expressed ER-α, ER-β, PR-(A+B), PR-B, VEGF, LIF, IL-1beta and COX-2. Epithelial cells expressed AR, integrinαVβ3 and MUC1. Mifepristone inhibited blastocyst attachment, up-regulated epithelial ER-β, PR-B; down regulated stromal VEGF, MUC1 and integrin αVβ3 as observed in vivo.

Conclusion:  An in vitro human embryo implantation model, regulated by progesterone was developed and tested. This could be further used to understand molecular basis of human embryo implantation.

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