ORIGINAL ARTICLE: Development of Human Graafian Follicles Following Transplantation of Human Ovarian Tissue into NOD/SCID/γcnull Mice


Yukihiro Terada, Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Seryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan.
E-mail: terada@mail.tains.tohoku.ac.jp


Problem  Transplantation of human ovarian cortex into host mice may permit various kinds of challenges in reproductive medicine. A novel immunodeficient mouse strain (NOD/SCID/γcnull: NOG) has been developed as a host of transplantation of human tissue.

Method of study  Human ovarian cortex was transplanted into various sites of NOG mice and human follicular development was examined by immunohistochemistry.

Results  Transplantation of human ovarian tissue into NOG mice resulted in approximately similar tissue survival and follicle growth as did transplantation into non-obese diabetic-severe combined immunodeficient mice. The human Graafian follicule from NOG mouse expressed the same steroidogenic enzymes as observed in human Graafian follicles, which developed in the human body. The NOG mice’s ovarian bursa was better placed for transplantation than the back skin or kidney capsule.

Conclusion  These results represent the successful generation and biological confirmation of the human Graafian follicles from the human ovarian cortex in the NOG mice.