ORIGINAL ARTICLE: Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with β2-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry
Article first published online: 11 DEC 2008
© 2009 The Authors. Journal compilation © 2009 Blackwell Munksgaard
American Journal of Reproductive Immunology
Volume 61, Issue 1, pages 39–51, January 2009
How to Cite
Inic-Kanada, A., Stojanovic, M., Zivkovic, I., Kosec, D., Micic, M., Petrusic, V., Zivancevic-Simonovic, S. and Dimitrijevic, L. (2009), ORIGINAL ARTICLE: Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross-Reacts with β2-Glycoprotein I: Its Characteristics and Role in Molecular Mimicry. American Journal of Reproductive Immunology, 61: 39–51. doi: 10.1111/j.1600-0897.2008.00660.x
- Issue published online: 11 DEC 2008
- Article first published online: 11 DEC 2008
- Submitted June 10, 2008; accepted September 19, 2008.
- Antiphospholipid syndrome;
- fetal loss;
- molecular mimicry;
- tetanus toxoid;
- β2-glycoprotein I
Problem Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein β2-glycoprotein I (β2GPI) and structure within micro-organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against β2GPI.
Method of study In this report, we analysed the characteristics of MAb26 raised against TTd and cross-reactive with β2GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo.
Results We have demonstrated that MAb26: (i) binds β2GPI being immobilized on an appropriate surface: irradiated polystyrene plates, non-irradiated plates pre-coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is β2GPI-dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice.
Conclusion This study concluded that certain subpopulations of antibodies raised against TTd and cross-reactive with β2GPI, because of the molecular mimicry mechanism, could have pathologic potential.