ORIGINAL ARTICLE: Effect of Progesterone on HLA-E Gene Expression in JEG-3 Choriocarcinoma Cell Line
Article first published online: 12 FEB 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 61, Issue 3, pages 221–226, March 2009
How to Cite
Huang, Z., Hyodo, H., Fujii, T., Nagamatsu, T., Matsumoto, J., Kawana, K., Yamashita, T., Yasugi, T., Kozuma, S. and Taketani, Y. (2009), ORIGINAL ARTICLE: Effect of Progesterone on HLA-E Gene Expression in JEG-3 Choriocarcinoma Cell Line. American Journal of Reproductive Immunology, 61: 221–226. doi: 10.1111/j.1600-0897.2008.00684.x
- Issue published online: 12 FEB 2009
- Article first published online: 12 FEB 2009
- Submitted November 22, 2008; accepted December 29, 2008.
- human leukocyte antigen-E;
Problem Among class Ib human leukocyte antigen (HLA) molecules, HLA-E is known to be a major ligand of CD94/NKG2 receptor on natural killer (NK) cells, and to play a pivotal role in recognition of extravillous trophoblasts (EVTs) by maternal immune cells. However, it is scarcely known how HLA-E expression is regulated in EVTs.
Method of study In this study, we investigated whether progesterone, an essential hormone in maintaining pregnancy, regulated HLA-E expression in EVT-like cell line, JEG-3. HLA-E mRNA amount in cultured JEG-3 cells was assessed by real-time PCR and cell-surface HLA-E protein was analyzed by flowcytometry.
Results Real-time PCR showed 3.5-fold increase 1 hour after the addition of 1000 ng/ml progesterone. This response was dimished by the addition of RU486, an antagonist for progesterone receptor. Flowcytometry indicated that 1000 ng/ml progesterone slightly enhanced HLA-E expression on the surface of JEG-3.
Conclusion These results suggest that progesterone up-regulates HLA-E expression in JEG-3 cells through the pathway mediated by progesterone receptor. Our findings might give a new insight into immunomodulatory function of progesterone at fetomaternal interface.