ORIGINAL ARTICLE: Sperm Antibodies, Intra-Acrosomal Sperm Proteins, and Cytokines in Semen in Men from Infertile Couples
Article first published online: 12 FEB 2009
© 2009 The Authors. Journal compilation © 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 61, Issue 3, pages 236–245, March 2009
How to Cite
Ulcova-Gallova, Z., Gruberova, J., Vrzalova, J., Bibkova, K., Peknicova, J., Micanova, Z. and Topolcan, O. (2009), ORIGINAL ARTICLE: Sperm Antibodies, Intra-Acrosomal Sperm Proteins, and Cytokines in Semen in Men from Infertile Couples. American Journal of Reproductive Immunology, 61: 236–245. doi: 10.1111/j.1600-0897.2009.00686.x
- Issue published online: 12 FEB 2009
- Article first published online: 12 FEB 2009
- Submitted September 10, 2008; accepted January 6, 2009
- intra-acrosomal proteins;
- male infertility;
- sperm antibodies
Problem The aim of this study was to investigate seminal sperm-agglutinating antibodies, intra-acrosomal proteins, sperm head abnormalities, and cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70 TNF-α, and IFN-γ) in men from infertile couples.
Method of study The direct mixed anti-immunoglobulin reaction test for IgG, IgA, and IgE in semen, and immunocytochemical method using monoclonal antibodies and indirect immunofluorescence for the examination of intra-acrosomal proteins in the spermatozoa were used. Cytokines in seminal plasma were determined by multiplex immunoanalytic xMAP (LUMINEX) technology.
Results Sperm-agglutinating antibodies, IgG and IgA, in seminal plasma were found to be more in asthenospermatic and oligoasthenospermatic men than in normospermatic men. Sperm head pathology and very low amounts of acrosomal proteins were frequently detected in pathologic semen samples. Cytokine levels defined as ‘high’ (based on the 75 percentile for each cytokine in all groups) were obtained especially for IL-8, IL-5, IL-6, and IL-10. The high cellularity in semen was correlated with higher IL-5.
Conclusion Immunologic cause of male infertility is a very important risk factor in the pathogenesis of sperm cells. Sperm autoantibodies and the presence of intra-acrosomal factors must be studied together, cytokines according to accessory cellularity in the semen.