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Keywords:

  • Abortion;
  • CD36;
  • macrophage;
  • TSP-1

Problem  To investigate the role of TSP-1 on decidual macrophages (DMΦ) in unexplained recurrent spontaneous abortion (RSA).

Method of study  A total of 20 women undergoing artificial abortion in the first trimester and 10 patients with RSA were selected. (i) The expression of TSP-1 mRNA in deciduas was detected by real-time polymerase chain reaction; (ii) Flow cytometry was used to detect the percentage of positive TSP-1, CD36, CD47 markers on macrophages. (iii) Cytokine expression was measured by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay.

Results  (i) The expression of TSP-1 mRNA on DMΦ in RSA was significantly decreased (< 0.05). (ii) The increased expression of CD36 (P < 0.01) and the decreased expression of TSP1 (P < 0.01) were found on DMΦ of RSA. No different CD47 expression level on DMΦ was observed between two groups. (iii)The expression of IL-10 in DMΦ of RSA patients was decreased significantly compared with that of controls (P < 0.05). When adding TSP1 into culture medium, there was no change in IFN-γ expression, but increased IL-10 (P < 0.05) expression in DMΦ of RSA patients was observed. The expression of IL-10 was decreased significantly in DMΦ from controls when adding anti-TSP-1 antibody to culture medium (P < 0.05).

Conclusion  TSP-1 on DMΦ could influence IL-10 expression as Th2 cytokines. The abnormal expression of TSP-1 could make some women undergo pregnancy loss.