They are the first co-authors contributing to this paper.
ORIGINAL ARTICLE: The Role of TSP-1 on Decidual Macrophages Involved in the Susceptibility to Unexplained Recurrent Spontaneous Abortion
Article first published online: 12 FEB 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 61, Issue 3, pages 253–260, March 2009
How to Cite
Jin, Y., Wang, X., Xiao, Y., Lv, C., Ding, C. and Lin, Q. (2009), ORIGINAL ARTICLE: The Role of TSP-1 on Decidual Macrophages Involved in the Susceptibility to Unexplained Recurrent Spontaneous Abortion. American Journal of Reproductive Immunology, 61: 253–260. doi: 10.1111/j.1600-0897.2009.00689.x
- Issue published online: 12 FEB 2009
- Article first published online: 12 FEB 2009
- Submitted October 17, 2008; accepted January 16, 2009.
Problem To investigate the role of TSP-1 on decidual macrophages (DMΦ) in unexplained recurrent spontaneous abortion (RSA).
Method of study A total of 20 women undergoing artificial abortion in the first trimester and 10 patients with RSA were selected. (i) The expression of TSP-1 mRNA in deciduas was detected by real-time polymerase chain reaction; (ii) Flow cytometry was used to detect the percentage of positive TSP-1, CD36, CD47 markers on macrophages. (iii) Cytokine expression was measured by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay.
Results (i) The expression of TSP-1 mRNA on DMΦ in RSA was significantly decreased (P < 0.05). (ii) The increased expression of CD36 (P < 0.01) and the decreased expression of TSP1 (P < 0.01) were found on DMΦ of RSA. No different CD47 expression level on DMΦ was observed between two groups. (iii)The expression of IL-10 in DMΦ of RSA patients was decreased significantly compared with that of controls (P < 0.05). When adding TSP1 into culture medium, there was no change in IFN-γ expression, but increased IL-10 (P < 0.05) expression in DMΦ of RSA patients was observed. The expression of IL-10 was decreased significantly in DMΦ from controls when adding anti-TSP-1 antibody to culture medium (P < 0.05).
Conclusion TSP-1 on DMΦ could influence IL-10 expression as Th2 cytokines. The abnormal expression of TSP-1 could make some women undergo pregnancy loss.