ORIGINAL ARTICLE: TNFα Gene Silencing Reduced Lipopolysaccharide-Promoted Proliferation of Endometriotic Stromal Cells
Article first published online: 2 MAR 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 61, Issue 4, pages 277–285, April 2009
How to Cite
Miyamoto, A., Taniguchi, F., Tagashira, Y., Watanabe, A., Harada, T. and Terakawa, N. (2009), ORIGINAL ARTICLE: TNFα Gene Silencing Reduced Lipopolysaccharide-Promoted Proliferation of Endometriotic Stromal Cells. American Journal of Reproductive Immunology, 61: 277–285. doi: 10.1111/j.1600-0897.2009.00691.x
- Issue published online: 2 MAR 2009
- Article first published online: 2 MAR 2009
- Submitted November 6, 2008; accepted January 21, 2009.
- cDNA microarray;
- cell proliferation;
- TNFα siRNA
Problem We previously reported that lipopolysaccharide (LPS)-promoted endometriotic stromal cell (ESC) proliferation by inducing TNFα production. The aim of this study was to investigate the efficacy of TNFα gene silencing on LPS-treated ESCs.
Method of study Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS-induced gene expression profiling after transfection of TNFα siRNA into ESCs was performed. Down-regulated genes by TNFα silencing were examined using real-time RT-PCR. Effect of TNFα silencing was examined using ELISA and BrdU incorporation, respectively.
Results In PCR array, TNFα silencing in ESCs repressed LPS-induced expression of cIAP2 and IL-8, NFκB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL-8 expression in ESCs were higher compared with those in EMSCs. TNFα silencing attenuated the LPS-induced ESC proliferation.
Conclusion Tumor necrosis factor α may be involved in cell proliferation of endometriotic tissues.