ORIGINAL ARTICLE: A High Dose of Intravenous Immunoglobulin Increases CD94 Expression on Natural Killer Cells in Women with Recurrent Spontaneous Abortion

Authors

  • Shigeki Shimada,

    1. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    2. Women’s Health Educational System, Hokkaido University Graduate School of Medicine, Sapporo, Japan
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  • Masamitsu Takeda,

    1. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
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  • Jun Nishihira,

    1. Department of Medical Management and Informatics, Hokkaido Information University, Ebetsu, Japan
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  • Masanori Kaneuchi,

    1. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    2. Women’s Health Educational System, Hokkaido University Graduate School of Medicine, Sapporo, Japan
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  • Noriaki Sakuragi,

    1. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    2. Women’s Health Educational System, Hokkaido University Graduate School of Medicine, Sapporo, Japan
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  • Hisanori Minakami,

    1. Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
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  • Hideto Yamada

    1. Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan
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Hideto Yamada, Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho Chuo-ku, Kobe 650-0017, Japan.
E-mail: yhideto@med.kobe-u.ac.jp

Abstract

Problem  A high dose of intravenous immunoglobulin (HIVIg) therapy is effective in various diseases such as autoimmune diseases, and also is expected to have efficacy in recurrent spontaneous abortion (RSA). The aim of this study was to understand immunological mechanisms of this therapy.

Method of study  By flowcytometric analyses, we examined phenotypic changes of a variety of immunological cells including natural killer (NK) cells, cytotoxic T cells, regulatory T cells and macrophages in peripheral blood of RSA women with HIVIg therapy (n = 8).

Results  Expression percentages of inhibitory CD94 on NK cells significantly (P = 0.01) increased after the therapy (58.8 ± 21.4% versus 71.0 ± 17.6%).

Conclusion  Mechanisms of possible efficacy of HIVIg therapy for RSA may include enhancement of CD94 expression and subsequent suppression of NK cell cytotoxicity.

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