ORIGINAL ARTICLE: Correlation Between Natural Cytotoxicity Receptors and Intracellular Cytokine Expression of Peripheral Blood NK Cells in Women with Recurrent Pregnancy Losses and Implantation Failures
Version of Record online: 11 OCT 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 62, Issue 6, pages 371–380, December 2009
How to Cite
Fukui, A., Ntrivalas, E., Fukuhara, R., Fujii, S., Mizunuma, H., Gilman-Sachs, A., Beaman, K. and Kwak-Kim, J. (2009), ORIGINAL ARTICLE: Correlation Between Natural Cytotoxicity Receptors and Intracellular Cytokine Expression of Peripheral Blood NK Cells in Women with Recurrent Pregnancy Losses and Implantation Failures. American Journal of Reproductive Immunology, 62: 371–380. doi: 10.1111/j.1600-0897.2009.00750.x
- Issue online: 6 NOV 2009
- Version of Record online: 11 OCT 2009
- Submitted March 13, 2009; accepted August 26, 2009.
- natural cytotoxicity receptors;
- NK cytokines;
- NK cell;
Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. We investigated whether women with recurrent pregnancy losses (RPLs) and implantation failures have aberrant correlation between NCRs and intracellular cytokine expression of NK cells.
Method of study Peripheral blood NK cells (CD56dim and CD56bright) were analyzed for NCRs (NKp46, NKp44 and NKp30) and cytokine expression (TNF-α, IFN-γ, IL-4, IL-10) using flow cytometry in RPL (n = 22), implantation failures (n = 23) or controls (n = 15).
Results In type 1 cytokine studies, CD56bright/NKp30+ cells in controls (r = 0.696, P < 0.05) were positively correlated with CD56bright/IFN-γ+/TNF-α+ cells. CD56bright/NKp46+ cells in implantation failures (r = −0.76, P < 0.01) were negatively correlated with CD56bright/IFN-γ+/TNF-α− cells. RPL did not have any correlation. In type 2 cytokine studies, CD56+/NKp46+ cells (r = 0.758, P < 0.01) and CD56+/NKp30+ cells (r = 0.637, P < 0.05) were positively correlated with CD56bright/IL-4+/IL-10+ cells in controls. CD56+/NKp30+ cells in implantation failures (r = −0.778, P < 0.05) were negatively correlated with CD56bright/IL-10+/IL-4+ cells. There were no correlations in RPL.
Conclusion Recurrent pregnancy losses and implantation failures have lack of, or negative correlation between NCRs and intracellular cytokines expression. This observation suggests that excessive pro-inflammatory cytokine expression in NK cells in RPL and implantation failures may be exerted through the NCRs or interruption of signal transduction processes.