Present Address: Assisted Human Reproduction Implementation Office, Health Canada, 2nd Floor, Room 350-30, Place du Centre, 200 Promenade du Portage, Gatineau, Canada K1A 0K9.
ORIGINAL ARTICLE: Pregnancy Does not Deter the Development of a Potent Maternal Protective CD8+ T-Cell Acquired Immune Response Against Listeria Monocytogenes Despite Preferential Placental Colonization
Article first published online: 12 NOV 2009
© 2009 Crown in the right of Canada
American Journal of Reproductive Immunology
Volume 63, Issue 1, pages 54–65, January 2010
How to Cite
Krishnan, L., Pejcic-Karapetrovic, B., Gurnani, K., Zafer, A. and Sad, S. (2010), ORIGINAL ARTICLE: Pregnancy Does not Deter the Development of a Potent Maternal Protective CD8+ T-Cell Acquired Immune Response Against Listeria Monocytogenes Despite Preferential Placental Colonization. American Journal of Reproductive Immunology, 63: 54–65. doi: 10.1111/j.1600-0897.2009.00766.x
- Issue published online: 14 DEC 2009
- Article first published online: 12 NOV 2009
- Submitted July 10, 2009; accepted September 18, 2009.
- Acquired immunity;
- CD8 T-cell response;
Listeria monocytogenes (LM) preferentially colonizes the placenta and causes fetal loss and systemic disease during pregnancy. As systemic CD8+ T-cell memory is critical in controlling LM infection, we addressed the issue as to whether it is modulated during pregnancy.
Method of study
Pregnant mice were infected with LM and their immune response was quantified relative to the non-pregnant cohort using advanced immunological techniques.
Pregnant mice exhibited progressive and massive placental LM infection leading to fetal resorptions. In contrast, they harbored significantly lower bacteria in spleen and liver relative to non-pregnant controls, and rapidly cleared systemic infection. Both pregnant and non-pregnant mice exhibited similar activation of systemic innate immunity. Moreover, LM infection in pregnant and non-pregnant hosts evoked strong antigen-specific cytolytic CD8+ T cells that produced IFN-γ. Consequently, LM infection initiated during pregnancy afforded long-term protective memory to secondary infection.
Maternal hosts generate a normal Listeria-specific adaptive immunity in particular CD8+ T-cell memory response suggesting that systemic listeriosis during pregnancy may be an immunopathology associated with placental infection.