ORIGINAL ARTICLE: Association Study of Vascular Endothelial Growth Factor and Polymorphisms of its Gene with Ectopic Pregnancy
Article first published online: 15 DEC 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 63, Issue 2, pages 120–125, February 2010
How to Cite
Elito, J., Daher, S., Fernandes da Silva, M. O., Marconi, N. M. H., Pendeloski, K. P. T., Moron, A. F. and Camano, L. (2010), ORIGINAL ARTICLE: Association Study of Vascular Endothelial Growth Factor and Polymorphisms of its Gene with Ectopic Pregnancy. American Journal of Reproductive Immunology, 63: 120–125. doi: 10.1111/j.1600-0897.2009.00769.x
- Issue published online: 6 JAN 2010
- Article first published online: 15 DEC 2009
- Submitted August 20, 2009; accepted September 30, 2009.
- Ectopic pregnancy;
Citation Elito J Jr, Daher S, Fernandes da Silva MO, Marconi NMH, Pendeloski KPT, Moron AF, Camano L. Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy. Am J Reprod Immunol 2010; 63: 120–125
Problem In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between −634C/G, −460T/C, and +936C/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.
Method of study This is a case–control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post-menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (−634C/G, −460T/C, and +936C/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.
Results The −634C/G, −460T/C, and +936C/T VEGF polymorphisms were not associated with ectopic pregnancy (P = 0.170, P = 0.285, and P = 0.700, respectively). The serum levels of VEGF were not associated with the genotype of −634C/G, −460T/C, and +936C/T VEGF polymorphisms (P = 0.702; P = 0.347, and P = 0.256, respectively).
Conclusion There was no association between ectopic pregnancy and −634C/G, −460T/C, and +936C/T VEGF polymorphisms. There was no correlation between VEGF genotype and the expression of VEGF in blood samples.