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Keywords:

  • intrauterine infection;
  • pre-term birth;
  • sulfasalazine

Citation Nath CA, Ananth CV, Smulian JC, Peltier MR. Can sulfasalazine prevent infection-mediated pre-term birth in a murine model? Am J Reprod Immunol 2010; 63: 144–149

Problem  Sulfasalazine (SASP) blocks activation of nuclear factor-kappa B (NF-κB) in gestational tissues in vitro– one of the earliest signals in the inflammatory response. We hypothesized that the administration of SASP would reduce the rate of infection-mediated pre-term birth in a murine model.

Method of study  CD-1 mice (n = 40) were assigned on gestational day (gd) 14.5 to 1 of 3 treatments: (1) Sham infection and vehicle; (2) 104 CFU Escherichia coli and vehicle; or (3) 104 CFU E. coli and SASP (150 mg/Kg daily). Mice were observed twice daily and deliveries prior to gd 18.5 were considered pre-term.

Results  Significantly more mice delivered prior to gd 18.5 when infected with 104 CFU E. coli than sham-infected mice (P < 0.001) and this effect was significantly reduced in mice also treated with SASP (P = 0.002). SASP also tended to increase litter size (P = 0.060) and significantly increased weight of pups born to dams with intrauterine infections (P = 0.001).

Conclusion  SASP reduced rates of pre-term delivery and improved pregnancy outcomes for mice infected with 104 CFU E. coli. This suggests that SASP has the potential to play a role in strategies to prevent pre-term birth in women.