ORIGINAL ARTICLE: Can Sulfasalazine Prevent Infection-Mediated Pre-Term Birth in a Murine Model?
Article first published online: 20 DEC 2009
© 2009 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 63, Issue 2, pages 144–149, February 2010
How to Cite
Nath, C. A., Ananth, C. V., Smulian, J. C. and Peltier, M. R. (2010), ORIGINAL ARTICLE: Can Sulfasalazine Prevent Infection-Mediated Pre-Term Birth in a Murine Model?. American Journal of Reproductive Immunology, 63: 144–149. doi: 10.1111/j.1600-0897.2009.00773.x
- Issue published online: 6 JAN 2010
- Article first published online: 20 DEC 2009
- Submitted June 29, 2009; accepted October 2, 2009.
- intrauterine infection;
- pre-term birth;
Citation Nath CA, Ananth CV, Smulian JC, Peltier MR. Can sulfasalazine prevent infection-mediated pre-term birth in a murine model? Am J Reprod Immunol 2010; 63: 144–149
Problem Sulfasalazine (SASP) blocks activation of nuclear factor-kappa B (NF-κB) in gestational tissues in vitro– one of the earliest signals in the inflammatory response. We hypothesized that the administration of SASP would reduce the rate of infection-mediated pre-term birth in a murine model.
Method of study CD-1 mice (n = 40) were assigned on gestational day (gd) 14.5 to 1 of 3 treatments: (1) Sham infection and vehicle; (2) 104 CFU Escherichia coli and vehicle; or (3) 104 CFU E. coli and SASP (150 mg/Kg daily). Mice were observed twice daily and deliveries prior to gd 18.5 were considered pre-term.
Results Significantly more mice delivered prior to gd 18.5 when infected with 104 CFU E. coli than sham-infected mice (P < 0.001) and this effect was significantly reduced in mice also treated with SASP (P = 0.002). SASP also tended to increase litter size (P = 0.060) and significantly increased weight of pups born to dams with intrauterine infections (P = 0.001).
Conclusion SASP reduced rates of pre-term delivery and improved pregnancy outcomes for mice infected with 104 CFU E. coli. This suggests that SASP has the potential to play a role in strategies to prevent pre-term birth in women.