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ORIGINAL ARTICLE: Profile of Peripheral Blood Neutrophil Cytokines in Diabetes Type 1 Pregnant Women and its Correlation with Selected Parameters in the Newborns


Małgorzata Sobczak, Outpatient Clinic of Foetal-Maternal Medicine and Gynaecology Department, Polish Mother’s Memorial Hospital Research Institute, Rzgowska Str 281/289, 93-338 Lodz, Poland.


Citation Pertyńska-Marczewska M, Głowacka E, Grodzicka A, Sobczak M, Cypryk K, Wilczyński JR., Wilczyński J. Profile of peripheral blood neutrophil cytokines in diabetes type 1 pregnant women and its correlation with selected parameters in the newborns. Am J Reprod Immunol 2010; 63: 150–160

Problem  Interleukin (IL)-12, IL-10, tumor necrosis factor-α (TNF-α), IL-6 and IL-8 alter as pregnancy progresses, implying continuous immune regulation associated with the maintenance of pregnancy. We aimed to evaluate the peripheral blood neutrophil-derived production of these cytokines in the course of pregnancy complicated by type 1 diabetes.

Method of study  These parameters were measured in samples from healthy non-pregnant (C), diabetic non-pregnant (D), healthy pregnant (P) and pregnant diabetic (PD) women.

Results  Neutrophil-derived secretion of TNF-α and IL-12 increased along with progression of pregnancy in PD and P groups. The concentration of IL-10 from lipopolysaccharide (LPS)-stimulated neutrophils increased during the course of uncomplicated pregnancy but decreased in diabetic pregnancy. Concentration of IL-8 decreased with the advancing gestational age in P and PD groups. LPS-stimulated neutrophil-derived IL-6 concentration increased only in PD patients.

Conclusion  Our results show that diabetes creates pro-inflammatory environment thus potentially influencing the outcome of pregnancy. We conclude that neutrophil-derived cytokine production could contribute to the complications seen in pregnant women with type 1 diabetes.