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ORIGINAL ARTICLE: Immunocontraceptive Effect of DNA Vaccine Targeting Fertilin β in Male Mice

Authors

  • Zhongyi Sun,

    1. Department of Urology, Third Affiliated (Daping) Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing City, China
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  • Fengshuo Jin,

    1. Department of Urology, Third Affiliated (Daping) Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing City, China
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  • Yanfeng Li,

    1. Department of Urology, Third Affiliated (Daping) Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing City, China
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  • Jun Zhang

    1. Department of Urology, Third Affiliated (Daping) Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing City, China
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Fengshuo Jin, Department of Urology, Third Affiliated (Daping) Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing City 400042, China.
E-mail: sun197699@hotmail.com

Abstract

Citation Sun Z, Jin F, Li Y, Zhang J. Immunocontraceptive effect of DNA vaccine targeting fertilin β in male mice. Am J Reprod Immunol 2010; 63: 282–290

Problem  In previous study, two eukaryotic expression plasmids pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD were successfully constructed and transfected in HEK293 cells. Now, we want to evaluate the immunocontraceptive effect of these two DNA vaccines that target the extracellular domain (Fβ.ECD) of sperm antigen fertilin β subunit in Kunming male mice.

Method of study  DNA vaccines pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD were injected into Kunming male mice three times at 0, 4, and 8 weeks, respectively. An antifertility effect was observed. Serum antibody and cytokines were also detected.

Results  Both vaccines significantly decreased both the pregnancy rate and the number of newborns. The serum levels of IL-2 and INF-γ significantly decreased, whereas the levels of IL-4 and IL-10 significantly increased. Compared with pSG.SS.YL-Fβ.ECD, pSG.SS.C3d3.YL-Fβ.ECD was more effective in birth control, and its specific Fβ-IgG antibody titer in serum was significantly higher and longer.

Conclusion  The results indicate that both pSG.SS.YL-Fβ.ECD and pSG.SS.C3d3.YL-Fβ.ECD DNA vaccines are effective in birth control of mice. The immunocontraceptive effect of Fβ.ECD DNA vaccine in male mice is improved with the addition of immuno-adjuvant C3d3.

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