These authors contributed equally to this work.
ORIGINAL ARTICLE: The Persistence of Paternal Antigens in the Maternal Body is Involved in Regulatory T-Cell Expansion and Fetal-Maternal Tolerance in Murine Pregnancy
Article first published online: 4 JAN 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 63, Issue 3, pages 200–208, March 2010
How to Cite
Zenclussen, M. L., Thuere, C., Ahmad, N., Wafula, P. O., Fest, S., Teles, A., Leber, A., Casalis, P. A., Bechmann, I., Priller, J., Volk, H.-D. and Zenclussen, A. C. (2010), ORIGINAL ARTICLE: The Persistence of Paternal Antigens in the Maternal Body is Involved in Regulatory T-Cell Expansion and Fetal-Maternal Tolerance in Murine Pregnancy. American Journal of Reproductive Immunology, 63: 200–208. doi: 10.1111/j.1600-0897.2009.00793.x
- Issue published online: 8 FEB 2010
- Article first published online: 4 JAN 2010
- Submitted November 5, 2009; accepted November 5, 2009.
- Fetal-maternal interface;
- male antigens;
- reproductive immunology;
- Treg cells
Citation Zenclussen ML, Thuere C, Ahmad N, Wafula PO, Fest S, Teles A, Leber A, Casalis PA, Bechmann I, Priller J, Volk H-D, Zenclussen AC. The persistence of paternal antigens in the maternal body is involved in regulatory T-cell expansion and fetal-maternal tolerance in murine pregnancy. Am J Reprod Immunol 2010; 63: 200–208
Problem Mammalian pregnancy is a state of immunological tolerance and CD4+ CD25+ regulatory T cells (Treg) contribute to its maintenance. Knowing that Treg act in an antigen-specific way during pregnancy, we hypothesized that they are generated after maternal immune cells encounter paternal antigens.
Method of study We mated wild type females with transgenic green fluorescent protein (GFP) males in an allogenic setting and killed them on different days of pregnancy.
Results Presence of paternal and maternal MHC class II+ cells in vaginal lavage on day 0.5 of pregnancy was confirmed. Thus, antigen presentation may take place early during pregnancy in the periphery either by the direct or indirect pathways. Foxp3+ cells known to have regulatory activity could be detected on day 2 of pregnancy in lymph nodes and shortly after implantation at the fetal-maternal interface.
Conclusion Our data suggest that paternal antigens are processed early during pregnancy, which leads to the generation of Treg. The continuous release of placental antigens into the maternal circulation allows the maintenance of a Treg population which is specific for paternal antigens and mediates tolerance toward the semi-allogeneic fetus until the time point of birth.