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ORIGINAL ARTICLE: Enhanced Maternal Anti-Fetal Immunity Contributes to the Severity of Hypertensive Disorder Complicating Pregnancy

Authors

  • Li-Ping Liu,

    1. Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    2. Wuhan Women & Children Medical Healthcare Center, Wuhan, China
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  • Wei Huang,

    1. Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, China
    3. Key Laboratory of Organ Transplantation, Ministry of Health, Wuhan, China
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  • Yue-Chao Lu,

    1. Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Ai-Hua Liao

    1. Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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Ai-Hua Liao, No. 13, Hangkong Rd., 430030 Wuhan, China.
E-mail: aihualiao@hotmail.com

Abstract

Citation Liu L-P, Huang W, Lu Y-C, Liao A-H. Enhanced maternal anti-fetal immunity contributes to the severity of hypertensive disorder complicating pregnancy. Am J Reprod Immunol 2010

Problem  The aim of this study was to evaluate how fetal monocyte activation and maternal anti-fetal antigen-specific antibody-secreting cells (ASC) affect the severity of hypertensive disorder complicating pregnancy (HDCP).

Method of study  Forty-six healthy third-trimester pregnant women and 20 patients with gestational hypertension, 20 with mild pre-ecalmpsia and another 20 with severe pre-eclampsia were included in the study. Interleukin-6 (IL-6) release from cord blood monocytes was examined by intracellular cytokine staining and flow cytometric analysis. Moreover, the maternal anti-fetal antigen-specific ASC were detected by enzyme-linked immunospot assay.

Results  A significantly increased percentage of IL-6-positive monocytes were detected in the cord blood of study groups compared with the controls (< 0.01). The percentage of IL-6-positive monocytes was increased as the disease progressed (< 0.05). There were more anti-fetal antigen-specific ASC in the study groups than those in the controls (< 0.001). Furthermore, the anti-fetal antigen-specific ASC showed difference in gestational hypertensive and severe pre-eclamptic groups (< 0.05).

Conclusion  We conclude that the fetal monocyte activation and the increase in maternal anti-fetal antigen-specific ASC were related to the incidence and severity of HDCP. These results provide both indirect and direct evidence for the occurrence of exaggerated maternal humoral immunity against the fetal antigens in HDCP.

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