ORIGINAL ARTICLE: Women with Multiple Implantation Failures and Recurrent Pregnancy Losses have Increased Peripheral Blood T Cell Activation

Authors

  • Kwang Moon Yang,

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    2. Department of Obstetrics and Gynecology, Cheil General Hospital & Women’s Healthcare Center, Kwandong University College of Medicine, Seoul, Korea
    Search for more papers by this author
  • Evangelos Ntrivalas,

    1. Department of Microbiology and Immunology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Hye Jin Cho,

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Na Young Kim,

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Kenneth Beaman,

    1. Department of Microbiology and Immunology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Alice Gilman-Sachs,

    1. Department of Microbiology and Immunology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Joanne Kwak-Kim

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    2. Department of Microbiology and Immunology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author

Joanne Kwak-Kim, MD, Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School/Rosalind Franklin University of Medicine and Science, 830 West End Ct. Suite 400, Vernon Hills, IL 60061, USA.
E-mail: Joanne.kwakkim@rosalindfranklin.edu

Abstract

Citation Yang KM, Ntrivalas E, Cho HJ, Kim NY, Beaman K, Gilman-Sachs A, Kwak-Kim J. Women with multiple implantation failures and recurrent pregnancy losses have increased peripheral blood T cell activation. Am J Reprod Immunol 2010

Problem  We aim to determine whether peripheral blood T cell activation is associated with repeated implantation failures or recurrent pregnancy losses (RPLs).

Method of study  Women with a history of repeated implantation failure (n = 18) or RPLs (n = 17) comprise the study group. Normal fertile women (n = 11) are included as controls. Proportion of activated peripheral blood T cells (CD69+, CD154+) and Th1/Th2 cell ratios are measured by flow cytometric analysis.

Results  Proportions (%) of CD4+/154+ of CD4+ and CD8+/154+ of CD8+ cells were significantly higher in study group than those of controls. Proportions (%) of CD3+/69+ of CD3+ cells and CD8+/69+ of CD8+ cells were significantly increased in study group compared to controls. Proportion (%) of CD4+/69+ cells significantly correlated with % CD4+/154+ cells (P = 0.003). Activated cytotoxic T cells (CD8+/154+, CD8+/69+) inversely correlated with INF-γ/IL-10 producing CD3+/4+ T cell ratios. Proportion of activated CD3+/8+/69 and CD3+/8+/154+ cells was inversely correlated with IFN-γ/IL-10 expressing CD3+/4+ T cell ratios.

Conclusion  Women with MIFs or RPLs have increased T cell activation in peripheral blood lymphocytes, and T cell suppressor activation seems to be associated with decreased Th1 immunity. Further studies on T cell activation may elucidate molecular mechanisms controlling Th1 effectors.

Ancillary