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Keywords:

  • cytokines;
  • extracellular matrix;
  • placenta

Citation Fontana VA, Sanchez M, Cebral E, Calvo JC. Interferon-γ inhibits metalloproteinase activity and cytotrophoblast cell migration. Am J Reprod Immunol 2010; 64: 20–26

Problem  Establishment of a successful pregnancy relies on a complex fetal–mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in implantation and could affect the success of pregnancy.

Method of study  Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-γ and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.

Results  Interferon-γ inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.

Conclusion  Our results correlate with previous reports from our laboratory indicating that IFN- γ is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion.