Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
Article first published online: 28 FEB 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 64, Issue 1, pages 20–26, July 2010
How to Cite
Fontana, V. A., Sanchez, M., Cebral, E. and Calvo, J. C. (2010), Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration. American Journal of Reproductive Immunology, 64: 20–26. doi: 10.1111/j.1600-0897.2010.00816.x
- Issue published online: 7 JUN 2010
- Article first published online: 28 FEB 2010
- Submitted January 6, 2010; accepted January 19, 2010.
- extracellular matrix;
Citation Fontana VA, Sanchez M, Cebral E, Calvo JC. Interferon-γ inhibits metalloproteinase activity and cytotrophoblast cell migration. Am J Reprod Immunol 2010; 64: 20–26
Problem Establishment of a successful pregnancy relies on a complex fetal–mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in implantation and could affect the success of pregnancy.
Method of study Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-γ and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.
Results Interferon-γ inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.
Conclusion Our results correlate with previous reports from our laboratory indicating that IFN- γ is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion.