Elevated NK Cell Cytotoxicity, CD158a Expression in NK Cells and Activated T Lymphocytes in Peripheral Blood of Women with IVF Failures
Article first published online: 17 MAR 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 64, Issue 1, pages 58–67, July 2010
How to Cite
Chernyshov, V. P., Sudoma, I. O., Dons’koi, B. V., Kostyuchyk, A. A. and Masliy, Y. V. (2010), Elevated NK Cell Cytotoxicity, CD158a Expression in NK Cells and Activated T Lymphocytes in Peripheral Blood of Women with IVF Failures. American Journal of Reproductive Immunology, 64: 58–67. doi: 10.1111/j.1600-0897.2010.00825.x
- Issue published online: 7 JUN 2010
- Article first published online: 17 MAR 2010
- Submitted November 16, 2009; accepted January 25, 2010.
- Activating markers in T lymphocytes;
- in vitro fertilization failures;
- natural killer cytotoxicity
Citation Chernyshov VP, Sudoma IO, Dons’koi BV, Kostyuchyk AA, Masliy YV. Elevated NK cell cytotoxicity, CD158a expression in NK cells and activated T lymphocytes in peripheral blood of women with IVF failures. Am J Reprod Immunol 2010; 64: 58–67
Problem The aim of this study was to evaluate the role of elevated natural killer cytotoxicity (NKc) in women with multiple implantation failures (IF) in vitro fertilization–embryo transfer (IVF–ET) cycles.
Methods of study Seventy-nine antiphospholipid antibodies-negative women with IF including 33 women with elevated NKc were selected for investigation. K-562 cell line was used to evaluate NKc. Lymphocyte subsets, intracellular cytokines [interferon (IFN)-γ, interleukin (IL)-4, tumour necrosis factor, IL-10], expression of activating markers [CD69, human leukocyte antigen (HLA)-DR], CD8, KIR (CD158a), CD95, and chemokine receptors (CXCR3, CCR4) were estimated by flow cytometry.
Results In women with IF, levels of NKc were higher than in IVF successful women. IF was associated with higher expression of CD8, CD158a, and HLA-DR in NK cells, activating markers in T lymphocytes, and lower levels of CCR4+ and IL-4+ T lymphocyte subsets. Predictive value of single elevated NKc for IVF success was 0.85, but addition of two other abnormal parameters resulted in its decrease to <0.39.
Conclusions Elevated NKc is negative factor, though not critical for implantation in IVF cycles. Immune mechanism of IVF failure includes not only elevated NKc but also some other factors, such as elevated expression of CD8 and CD158a on NK cells, T lymphocyte activation, and diminished T helper 2 parameters.