Trophoblast-Derived Exosomes Mediate Monocyte Recruitment and Differentiation
Article first published online: 15 JUN 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 1, pages 65–77, January 2011
How to Cite
Atay, S., Gercel-Taylor, C., Suttles, J., Mor, G. and Taylor, D. D. (2011), Trophoblast-Derived Exosomes Mediate Monocyte Recruitment and Differentiation. American Journal of Reproductive Immunology, 65: 65–77. doi: 10.1111/j.1600-0897.2010.00880.x
- Issue published online: 9 DEC 2010
- Article first published online: 15 JUN 2010
- Submitted February 26, 2010; accepted April 15, 2010
- pro-inflammatory environment;
Citation Atay S, Gercel-Taylor C, Suttles J, Mor G, Taylor DD. Trophoblast-derived exosomes mediate monocyte recruitment and differentiation. Am J Reprod Immunol 2011; 65: 65–77
Introduction Trophoblast cells have been demonstrated to regulate monocyte migration and differentiation, leading to pro-inflammatory profiles. Because trophoblast cells release exosomes with immunoregulatory properties, trophoblast-derived exosomes are proposed to ‘educate’ monocytes, creating a pro-inflammatory environment.
Method of study Exosomes were isolated from conditioned media of Swan71 cells by ultrafiltration and ultracentrifugation. Exosome-induced migration was assessed using a two-chamber system. Cytokine profiles were defined using cytokine arrays, and mRNA levels of affected cytokines were examined by qRT-PCR and ELISA.
Results Within 20 min, 8–10% of monocytes took up labeled exosomes isolated from Swan71 cells. Trophoblast-derived exosomes increased monocyte migration in a dose-dependent manner and produced significant increases in production of interleukin (IL)-1β, IL-6, Serpin-E1, granulocyte colony-stimulating factor, granulocyte/monocyte colony-stimulating factor, and tumor necrosis factor-α.
Conclusion This study presents the initial demonstration that trophoblast-derived exosomes are capable of recruiting and ‘educating’ monocytes to produce pro-inflammatory cytokine/chemokine profiles in a cell-contact-independent manner.