IL-11 Expression in Human Term Placental Macrophages
Article first published online: 13 AUG 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 4, pages 397–402, April 2011
How to Cite
Pavlov, O. V., Kramareva, N. L. and Selkov, S. A. (2011), IL-11 Expression in Human Term Placental Macrophages. American Journal of Reproductive Immunology, 65: 397–402. doi: 10.1111/j.1600-0897.2010.00909.x
- Issue published online: 4 MAR 2011
- Article first published online: 13 AUG 2010
- Submitted April 29, 2010; accepted July 6, 2010.
- intracellular production;
- placental macrophages;
Citation Pavlov OV, Kramareva NL, Selkov SA. IL-11 Expression in human term placental macrophages. Am J Reprod Immunol 2011; 65: 397–402
Problem IL-11 is a cytokine with pleiotropic activities, including gestational effects. Whereas IL-11 production by maternal reproductive tissues is extensively studied, there is poor information about IL-11 sources in the fetal counterpart of the maternal–fetal unit.
Method of study We investigated the expression of IL-11 in the purified human term placenta macrophages, using flow cytometry and immunoenzyme assay.
Results Intracellular IL-11 was detected in a substantial proportion of cultured CD68+ cells (median 38%). IL-11 secretion by the placental macrophages was observed after 23-hr cultivation (median 4.3 pg/105 cells). Stimulation with lipopolysaccharide did not significantly change both intracellular expression and secretion of the cytokine.
Conclusion Demonstrated IL-11 expression by placental macrophages suggests that non-trophoblast cells contribute to IL-11 production at the maternal–fetal interface and thus account for the reproductive effects of the cytokine.