IL-11 Expression in Human Term Placental Macrophages


Oleg V. Pavlov, Department of Immunology, D.O.Ott Institute for Obstetrics and Gynecology, 3 Mendeleev line, 199034, Saint-Petersburg, Russia.


Citation Pavlov OV, Kramareva NL, Selkov SA. IL-11 Expression in human term placental macrophages. Am J Reprod Immunol 2011; 65: 397–402

Problem  IL-11 is a cytokine with pleiotropic activities, including gestational effects. Whereas IL-11 production by maternal reproductive tissues is extensively studied, there is poor information about IL-11 sources in the fetal counterpart of the maternal–fetal unit.

Method of study  We investigated the expression of IL-11 in the purified human term placenta macrophages, using flow cytometry and immunoenzyme assay.

Results  Intracellular IL-11 was detected in a substantial proportion of cultured CD68+ cells (median 38%). IL-11 secretion by the placental macrophages was observed after 23-hr cultivation (median 4.3 pg/105 cells). Stimulation with lipopolysaccharide did not significantly change both intracellular expression and secretion of the cytokine.

Conclusion  Demonstrated IL-11 expression by placental macrophages suggests that non-trophoblast cells contribute to IL-11 production at the maternal–fetal interface and thus account for the reproductive effects of the cytokine.