Regulatory Τ-cell Differentiation Between Maternal and Cord Blood Samples in Pregnancies with Spontaneous Vaginal Delivery and with Elective Cesarian Section
Article first published online: 20 AUG 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 2, pages 173–179, February 2011
How to Cite
Bili, H., Fleva, A., Pados, G., Argyriou, T., Tsolakidis, D., Pavlitou, A. and Tarlatzis, B. C. (2011), Regulatory Τ-cell Differentiation Between Maternal and Cord Blood Samples in Pregnancies with Spontaneous Vaginal Delivery and with Elective Cesarian Section. American Journal of Reproductive Immunology, 65: 173–179. doi: 10.1111/j.1600-0897.2010.00910.x
- Issue published online: 6 JAN 2011
- Article first published online: 20 AUG 2010
- Submitted April 10, 2010; accepted July 6, 2010.
- CD4+/25high cells;
- γ/δ T-cells
Citation Bili H, Fleva A, Pados G, Argyriou T, Tsolakidis D, Pavlitou A, Tarlatzis BC. Regulatory T-cell differentiation between maternal and cord blood samples in pregnancies with spontaneous vaginal delivery and with elective cesarian section. Am J Reprod Immunol 2011; 65: 173–179
Problem The immunological mechanisms preventing fetal antigenic rejection during normal pregnancy and the extent to which the type of delivery influences lymphocyte reactions are elusive.
Method of study Maternal peripheral blood and neonatal umbilical cord blood (CB) was collected upon labor after vaginal delivery or cesarian section. Leukocytes were analyzed with flow cytometry, focusing on regulatory and γ/δ T-cells.
Results In CB from neonates delivered by vaginal delivery, natural killer cells were increased. On the other hand, in maternal blood, γ/δ T-cells were increased, and activated T-cells (cluster of differentiation [CD]4+/25dim/122+ cells) were decreased. Moreover, maternal blood presented increased levels of T regulatory cell subsets like CD4+/25high/45RO+, CD4+/25high/DR+, CD4+/25high/CD38+ and CD4+/25high/71+. In CB, CD19+, CD4+/25high/45RA+ and CD4+/25high/122+ cells were increased.
Conclusion The effect of delivery type on lymphocyte immunophenotype was minimal. Mothers’ and neonates’ lymphocyte subsets differed significantly. Mothers’ phenotype comprised significantly of lymphocytes involved in tolerance (memory and activated regulatory T-cells, γ/δ T-cells).