Cytokine Dysregulation in Early- and Late-Term Placentas from Feline Immunodeficiency Virus (FIV)-Infected Cats
Article first published online: 5 SEP 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 5, pages 480–491, May 2011
How to Cite
Scott, V. L., Boudreaux, C. E., Lockett, N. N., Clay, B. T. and Coats, K. S. (2011), Cytokine Dysregulation in Early- and Late-Term Placentas from Feline Immunodeficiency Virus (FIV)-Infected Cats. American Journal of Reproductive Immunology, 65: 480–491. doi: 10.1111/j.1600-0897.2010.00919.x
- Issue published online: 5 APR 2011
- Article first published online: 5 SEP 2010
- Submitted June 9, 2010; accepted August 3, 2010.
- feline immunodeficiency virus;
Citation Scott VL, Boudreaux CE, Lockett NN, Clay BT, Coats KS. Cytokine dysregulation in early- and late-term placentas from feline immunodeficiency virus (FIV)-infected Cats. Am J Reprod Immunol 2011; 65: 480–491
Problem Experimental infection of cats with FIV-B-2542 produces high rates of fetal infection and reproductive failure. We hypothesized that dysregulation of placental cytokine expression occurs in FIV-infected queens, and aberrant expression potentiates inflammation and impacts pregnancy outcome. Our purpose was to quantify expression of representative pro-inflammatory cytokines (IL-6, IL-12p35, and IL-1β), IL-10 (anti-inflammatory), and the chemokine SDF-1α in early- and late-term placental tissues.
Method of study Real-time reverse transcriptase PCR was used to measure gene expression in placental tissues.
Results Increased expression of IL-6 and IL-12p35 and decreased expression of IL-10 occurred in FIV-infected tissues at early pregnancy; at late gestation, IL-6 expression increased and IL-1β and SDF-1α decreased. At late pregnancy, IL-6 expression positively correlated with FIV load. IL-12:IL-10 ratios were higher in infected tissues at early, but not late pregnancy. Fetal non-viability accompanied decreased IL-12p35 and SDF-1α expression at both stages and decreased IL-12:IL-10 ratio at late pregnancy.
Conclusion FIV infection caused a pro-inflammatory placental microenvironment at early, but not late pregnancy.