Focal Increases of Fetal Macrophages in Placentas from Pregnancies with Histological Chorioamnionitis: Potential Role of Fibroblast Monocyte Chemotactic Protein-1
Article first published online: 19 NOV 2010
© 2010 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 5, pages 470–479, May 2011
How to Cite
Toti, P., Arcuri, F., Tang, Z., Schatz, F., Zambrano, E., Mor, G., Niven-Fairchild, T., Abrahams, V. M., Krikun, G., Lockwood, C. J. and Guller, S. (2011), Focal Increases of Fetal Macrophages in Placentas from Pregnancies with Histological Chorioamnionitis: Potential Role of Fibroblast Monocyte Chemotactic Protein-1. American Journal of Reproductive Immunology, 65: 470–479. doi: 10.1111/j.1600-0897.2010.00927.x
- Issue published online: 5 APR 2011
- Article first published online: 19 NOV 2010
- Submitted September 28, 2010; accepted accepted October 5, 2010.
- fetal macrophages;
- Hofbauer cells;
- monocyte chemotactic protein-1;
Citation Toti P, Arcuri F, Tang Z, Schatz F, Zambrano E, Mor G, Niven-Fairchild T, Abrahams VM, Krikun G, Lockwood CJ, Guller S. Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1. Am J Reprod Immunol 2011; 65: 470–479
Problem Histopathological chorioamnionitis (HCA) is caused by microbial-driven infiltration of leukocytes to the maternal-fetal interface resulting in adverse neonatal outcomes in a subset of pregnancies. The role of placental villus macrophages (i.e. Hofbauer cells, HBCs) in the pathophysiology of HCA is unelucidated.
Method of study The number of HBCs in human term placental villi in HCA and control groups was compared using immunohistochemistry. Levels of monocyte chemotactic protein (MCP-1) expression were measured in primary cultures of syncytioytrophoblasts (SCTs) and fibroblasts (FIBs) treated with bacterial compounds [lipopolysaccharide (LPS) and peptidoglycan] and pro-inflammatory cytokines (TNF-α and IL-1β) using ELISA and quantitative real-time PCR.
Results Immunohistochemistry revealed a focal increase in HBCs in HCA. Treatment of FIBs with LPS, IL-1β, and TNF-α significantly increased MCP-1 mRNA and protein expression. Conversely, MCP-1 mRNA and protein levels were virtually undetectable in treated and untreated SCTs.
Conclusion These results demonstrate cell-type-specific regulation of MCP-1 expression in human placenta. A model is presented in which bacterial products and inflammatory cytokines initiate a fibroblast-driven cytokine cascade resulting in recruitment of fetal monocytes to placenta which focally increases levels of HBCs in pregnancies complicated by HCA.