Previous presentation in event: Presented at the XXI European Congress of Perinatal Medicine, Istanbul, Turkey, September 10–13, 2008.
Amniotic Fluid Interleukin-1 Beta and Interleukin-6, but not Interleukin-8 Correlate with Microbial Invasion of the Amniotic Cavity in Preterm Labor
Article first published online: 9 JAN 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 6, pages 549–556, June 2011
How to Cite
Marconi, C., de Andrade Ramos, B. R., Peraçoli, J. C., Donders, G. G. G. and da Silva, M. G. (2011), Amniotic Fluid Interleukin-1 Beta and Interleukin-6, but not Interleukin-8 Correlate with Microbial Invasion of the Amniotic Cavity in Preterm Labor. American Journal of Reproductive Immunology, 65: 549–556. doi: 10.1111/j.1600-0897.2010.00940.x
- Issue published online: 25 APR 2011
- Article first published online: 9 JAN 2011
- Submitted September 13, 2010; accepted October 26, 2010.
- intra-amniotic infection;
- Mycoplasma hominis;
- Ureaplasma urealyticum
Citation Marconi C, Ramos BRA, Peraçoli JC, Donders GGG, Silva MG. Amniotic fluid interleukin-1 betaand interleukin-6, but not interleukin-8 correlate with microbial invasion of the amniotic cavity in preterm labor. Am J Reprod Immunol 2011; 65: 549–556
Problem We compared the frequency of intra-amniotic infection in preterm labor (PL) with women not in labor, and correlated infection with amniotic fluid (AF) cytokines. Detailed identification of species, especially mycoplasmata, was tried to improve our understanding of the pathogenesis of PL.
Method of study AF from 20 women with PL and 20 controls were evaluated. Infection was detected by PCR for Mycoplasma hominis, Ureaplasma urealyticum and 16S rRNA bacterial gene, which was cloned and sequenced for bacterial identification. Interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α levels were measured by ELISA.
Results Frequency of intra-amniotic infection is higher in PL (40.0%). Sequencing-based method identified Bacteroides fragilis, Prevotella bivia and Leptotrichia amnionii, in addition to Mycoplasma species detected by PCR. AF infection correlated with increased IL-1β and IL-6 levels.
Conclusion The frequency of intra-amniotic infection, especially M. hominis, in PL women who delivered with 7 days, is high and correlates with high IL-1β and IL-6 levels, but not IL-8.