Effect of Human Endometrial Stromal Cell-derived Conditioned Medium on Uterine Natural Killer (uNK) Cells’ Proliferation and Cytotoxicity


Lili Huang, MD, Women’s Hospital, School of Medicine, Zhejiang University, 1 Xueshi Road, Hangzhou 310006, China.
E-mail: fbhuanglili@163.com


Citation Chen Y, Zhuang Y, Chen X, Huang L. Effect of human endometrial stromal cell-derived conditioned medium on uterine natural killer (uNK) cells’ proliferation and cytotoxicity. Am J Reprod Immunol 2011; 65: 589–596

Problem  Human endometrial stromal cells are involved in the regulation of immune cell proliferation, apoptosis, differentiation, and function. In the endometrium, uNK cells are in close contact with stromal cells. The aim of the study was to investigate the effects of human endometrial stromal cells on uNK-cell proliferation and uNK-cell cytotoxicity.

Method of study  The conditioned medium was derived from the endometrial stromal cells in the proliferative phase, secretory phase, and early pregnancy. The effects of stromal cell-derived conditioned medium on uNK-cell proliferation and cytotoxicity were detected by mitochondrial lactate dehydrogenase-based MTS staining and flow cytometry.

Results  The stromal cell-derived conditioned medium in both secretory phase and early pregnancy significantly promoted uNK-cell proliferation. Compared with the control group, the uNK-cell cytotoxicity were significantly reduced by conditioned medium in the proliferative, secretory, and decidua groups, but there were no significant differences among these different physiological stages in the inhibiting ability.

Conclusion  Human endometrial stromal cells may be involved in the regulation of uNK-cell functions through influencing proliferation and cytolytic activity.