Intracellular Cytokine Production in Peripheral Blood Lymphocytes: A Comparison of Values in Infertile and Fertile Women
Version of Record online: 18 JAN 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 5, pages 466–469, May 2011
How to Cite
Horká, P., Jarošová, R., Malíčková, K., Janatková, I., Marečková, H., Zima, T. and Kalousová, M. (2011), Intracellular Cytokine Production in Peripheral Blood Lymphocytes: A Comparison of Values in Infertile and Fertile Women. American Journal of Reproductive Immunology, 65: 466–469. doi: 10.1111/j.1600-0897.2010.00959.x
- Issue online: 5 APR 2011
- Version of Record online: 18 JAN 2011
- Submitted November 6, 2010; accepted November 29, 2010.
- flow cytometry;
Citation Horká P, Jarošová R, Malíčková K, Janatková I, Marečková H, Zima T, Kalousová M. Intracellular cytokine production in peripheral blood lymphocytes: a comparison of values in infertile and fertile women. Am J Reprod Immunol 2011; 65: 466–469
Problem To analyze the relation of the fertility and pregnancy of women of childbearing age to the intracellular (IC) production of tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and interleukins 2 and 4 (IL-2 and IL-4).
Method of study Intracellular cytokine production in peripheral blood CD3+ CD4+ lymphocytes was analyzed by flow cytometry in 185 women being treated for infertility and 50 fertile women of childbearing age.
Results Infertile women have a significantly higher IC production of TNF-α, IFN-γ, IL-2, and IL-4 and higher ratios of TNF-α/IL-2, TNF-α/IL-4, and TNF-α/IFN-γ compared to the fertile women.
Conclusion Cytokines produced by Th lymphocytes are important in orchestrating the immune response during conception, and Th-cell dysregulation could be a reason for infertility.