Current address: Hospital Zonal Bariloche, (8400)Bariloche, Rio Negro, Republica Argentina.
Increased COX-2 Expression in Human Vaginal Epithelial Cells Exposed to Nonoxynol-9, a Vaginal Contraceptive Microbicide that Failed to Protect Women from HIV-1 Infection
Article first published online: 18 JAN 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 65, Issue 6, pages 569–577, June 2011
How to Cite
Zalenskaya, I. A., Cerocchi, O. G., Joseph, T., Donaghay, M. A., Schriver, S. D. and Doncel, G. F. (2011), Increased COX-2 Expression in Human Vaginal Epithelial Cells Exposed to Nonoxynol-9, a Vaginal Contraceptive Microbicide that Failed to Protect Women from HIV-1 Infection. American Journal of Reproductive Immunology, 65: 569–577. doi: 10.1111/j.1600-0897.2010.00964.x
- Issue published online: 25 APR 2011
- Article first published online: 18 JAN 2011
- Submitted September 20, 2010; accepted December 6, 2010.
Data S1. Methods.
Figure S1. COX-2 expression in Ect1/E6E7 (a) and End1/E6E7 (b) cells exposed to N-9 at indicated concentrations for 6 hr. COX-2 in VK2/E6E7 cells is shown as a reference. COX-2 was detected using anti-COX-2 antibody. Loading was monitored by β-actin.
Figure S2. C31G induced COX-2 expression in VK2/E6E7 cells. The cells were treated with increasing concentrations of C31G. COX-2 was detected using anti-COX-2 antibody. Loading was monitored by Ponceau S staining of the blot.
Figure S3. COX-2 expression in VK2 cells exposed to microbicide candidates – polyanions PRO2000 and cellulose sulfate (CS,) and to placebo, hydroxyethyl cellulose (HEC) applied at a concentration of 1 mg/mL for 6 hr. N-9 is shown as a positive control. Loading was monitored by β-actin.
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