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Data S1. Methods.

Figure S1. COX-2 expression in Ect1/E6E7 (a) and End1/E6E7 (b) cells exposed to N-9 at indicated concentrations for 6 hr. COX-2 in VK2/E6E7 cells is shown as a reference. COX-2 was detected using anti-COX-2 antibody. Loading was monitored by β-actin.

Figure S2. C31G induced COX-2 expression in VK2/E6E7 cells. The cells were treated with increasing concentrations of C31G. COX-2 was detected using anti-COX-2 antibody. Loading was monitored by Ponceau S staining of the blot.

Figure S3. COX-2 expression in VK2 cells exposed to microbicide candidates – polyanions PRO2000 and cellulose sulfate (CS,) and to placebo, hydroxyethyl cellulose (HEC) applied at a concentration of 1 mg/mL for 6 hr. N-9 is shown as a positive control. Loading was monitored by β-actin.

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AJI_964_sm_f2.JPG96KSupporting info item
AJI_964_sm_f3.JPG51KSupporting info item
AJI_964_sm_fig-legend.doc23KSupporting info item

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