Present address: Mandy Cromwell, Genzyme Corporation, 153 Second Ave, Waltham, MA, USA.
SIV-Specific CD8+ T Cells are Enriched in Female Genital Mucosa of Rhesus Macaques and Express Receptors for Inflammatory Chemokines
Article first published online: 12 JAN 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Special Issue: Sexual Transmission of HIV in the 21st Century
Volume 65, Issue 3, pages 242–247, March 2011
How to Cite
Cromwell, M. A., Carville, A., Mansfield, K., Klumpp, S., Westmoreland, S. V., Lackner, A. A. and Johnson, R. P. (2011), SIV-Specific CD8+ T Cells are Enriched in Female Genital Mucosa of Rhesus Macaques and Express Receptors for Inflammatory Chemokines. American Journal of Reproductive Immunology, 65: 242–247. doi: 10.1111/j.1600-0897.2010.00966.x
- Issue published online: 7 FEB 2011
- Article first published online: 12 JAN 2011
- Submitted December 9, 2010; accepted December 9, 2010.
- CD8+ T cell responses;
- mucosal immunity;
Citation Cromwell MA, Carville A, Mansfield K, Klumpp S, Westmoreland SV, Lackner AA, Johnson RP. SIV-specific CD8+ T cells are enriched in female genital mucosa of rhesus macaques and express receptors for inflammatory chemokines. Am J Reprod Immunol 2011; 65: 242–247
Problem Mucosal T lymphocyte responses in the female reproductive tract, the primary site of HIV transmission in women, may be critical for initial control of virus infection. In addition, characterization of genital immune responses to HIV will be important for the development of a vaccine capable of preventing infection by this route.
Method of study We analyzed lymphocytes isolated from vagina and cervix of chronically SIV-infected macaques for the frequency of SIV Gag tetramer-binding cells and expression of chemokine receptors.
Results We found that the frequency of SIV-specific CD8+ T cell responses was 3- to 30-fold higher in genital tissues than in peripheral blood. SIV-specific CD8+ T cells in genital tissues expressed high levels of CXCR3 and CCR5, chemokine receptors normally expressed on memory T cells that home to inflamed tissues. Cells expressing CXCR3 colocalized with its chemokine ligand CXCL9 [monokine induced by interferon gamma, MIG] in the vaginal lamina propria.
Conclusion These results indicate that the frequency of SIV-specific CD8+ T cells in the female genital mucosa is enriched compared with peripheral blood and provide initial information regarding the signals that direct recruitment of T cells to the female reproductive tract.