Circulating Cell-Derived Microparticles in Women with Pregnancy Loss

Authors

  • Jaume Alijotas-Reig,

    1. Ageing and Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d’Hebron University Hospital, Faculty of Medicine, Universitat Autònoma, Barcelona, Spain;
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  • Carles Palacio-Garcia,

    1. Flow Cytometry Unit, Haematology Service, Vall d’Hebron University Hospital, Universitat Autònoma, Barcelona, Spain;
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  • Immaculada Farran-Codina,

    1. High Obstetric Risk Unit, Obstetric Department, Vall d’Hebron University Hospital, Universitat Autònoma, Barcelona, Spain;
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  • Cristina Zarzoso,

    1. High Obstetric Risk Unit, Obstetric Department, Vall d’Hebron University Hospital, Universitat Autònoma, Barcelona, Spain;
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  • Luis Cabero-Roura,

    1. Obstetric Department, Vall d’Hebron University Hospital, Faculty of Medicine, Universitat Autònoma, Barcelona, Spain
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  • Miquel Vilardell-Tarres

    1. Ageing and Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d’Hebron University Hospital, Faculty of Medicine, Universitat Autònoma, Barcelona, Spain;
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Jaume Alijotas-Reig, Ageing and Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d’Hebron University Hospital, Barcelona, Spain. E-mails: 16297jar@comb.es; jalijotas@vhebron.net

Abstract

Citation
Alijotas-Reig J, Palacio-Garcia C, Farran-Codina I, Zarzoso C, Cabero-Roura L, Vilardell-Tarres M. Circulating cell-derived microparticles in women with pregnancy loss. Am J Reprod Immunol 2011; 66: 199–208

Problem  To analyze cell-derived microparticles (cMP) in pregnancy loss (PL), both recurrent miscarriages (RM) and unexplained fetal loss (UFL).

Method of study  Non-matched case–control study was performed at Vall d’Hebron Hospital. Cell-derived microparticles of 53 PL cases, 30 with RM, 16 with UFL, and 7 (RM + UFL), were compared to 38 healthy pregnant women. Twenty healthy non-pregnant women act as controls. Cell-derived microparticles were analyzed through flow cytometry. Results are given as total annexin (A5+), endothelial-(CD144+/CD31+ CD41−), platelet-(CD41+), leukocyte-(CD45+) and CD41− c-MP/μL of plasma. Antiphospholipid antibodies (aPLA) were analyzed according to established methods.

Results  Comparing PL versus healthy pregnant, we observed a significant endothelial cMP decrease in PL. When comparing RM subgroup with controls, we observed significant decreases in endothelial cMP. When comparing the PL positive for aPLA versus PL-aPLA-negative, no cMP numbering differences were seen.

Conclusion  Pregnancy loss seems to be related to endothelial cell activation and/or consumption. A relationship between aPLA and cMP could not be demonstrated.

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