Signal Transducer and Activator of Transcription 3 (STAT3) and Suppressor of Cytokine Signaling (SOCS3) Balance Controls Cytotoxicity and IL-10 Expression in Decidual-Like Natural Killer Cell Line NK-92
Article first published online: 9 MAR 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 66, Issue 4, pages 329–335, October 2011
How to Cite
Braunschweig, A., Poehlmann, T. G., Busch, S., Schleussner, E. and Markert, U. R. (2011), Signal Transducer and Activator of Transcription 3 (STAT3) and Suppressor of Cytokine Signaling (SOCS3) Balance Controls Cytotoxicity and IL-10 Expression in Decidual-Like Natural Killer Cell Line NK-92. American Journal of Reproductive Immunology, 66: 329–335. doi: 10.1111/j.1600-0897.2011.00989.x
- Issue published online: 9 SEP 2011
- Article first published online: 9 MAR 2011
- Submitted December 28, 2010; accepted January 18, 2011.
- decidual NK cells;
- natural killer cells;
Citation Braunschweig A, Poehlmann TG, Busch S, Schleussner E, Markert UR. Signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling 3 (SOCS3) balance controls cytotoxicity and IL-10 expression in decidual-like natural killer cell line NK-92. Am J Reprod Immunol 2011; 66: 329–335
Objectives In previous studies, we have shown that sHLA-G reduces cytotoxicity of decidual NK cells, which was dependent upon reduction in signal transducer and activator of transcription 3 (STAT3) and perforin. In this study, we aimed to confirm the role of STAT3 for induction of cytotoxicity and to analyze the regulative role of its antagonist suppressor of cytokine signaling 3 (SOCS3). Furthermore, the influence of both factors on cytokine expression should be analyzed.
Methods All experiments were performed on NK-92 cells. STAT3 and SOCS3 have been silenced using two different small interfering RNA sequences each. Silencing efficiency and STAT3 tyrosine phosphorylation have been analyzed by Western blotting. Cytotoxicity to K562 target cells has been assessed by flow cytometry. Expression of IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF-α, and TNF-β has been measured using cytometric bead arrays for flow cytometry.
Results STAT3 and SOCS3 have been successfully silenced. STAT3 silencing reduced cytotoxicity. SOCS3 silencing induced increase in STAT3 tyrosine phosphorylation and cytotoxicity. STAT3 silencing reduced IL-10 expression significantly, while SOCS3 silencing induced, also significantly, the opposite effect. The other cytokines were expressed at very low concentration or not constantly affected.
Conclusion STAT3 and SOCS3 are involved in regulation of NK cell cytotoxicity and IL-10 expression.