Increased Reactive Oxygen Species and Tumor Necrosis Factor-Alpha Production by Monocytes are Associated with Elevated Levels of Uric Acid in Pre-Eclamptic Women

Authors

  • Maria T. S. Peraçoli,

    1. Department of Microbiology and Immunology, Institute of Biociences, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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  • Camila F. Bannwart,

    1. Department of Ginecology and Obstetrics, Botucatu Medical School, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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  • Renata Cristofalo,

    1. Department of Microbiology and Immunology, Institute of Biociences, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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  • Vera T. Medeiros Borges,

    1. Department of Ginecology and Obstetrics, Botucatu Medical School, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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  • Roberto A. Araújo Costa,

    1. Department of Ginecology and Obstetrics, Botucatu Medical School, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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  • Steven S. Witkin,

    1. Department of Obstetrics and Gynecology, Division of Immunology and Infectious Diseases, Weill Medical College of Cornell University, New York, NY, USA
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  • José C. Peraçoli

    1. Department of Ginecology and Obstetrics, Botucatu Medical School, UNESP- São Paulo State University, Botucatu, São Paulo, Brazil
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Maria Terezinha S. Peraçoli, Department of Microbiology and Immunology, Institute of Biosciences, Rubião Junior s/n, UNESP, Botucatu, Sao Paulo CEP 18618-970, Brazil.
E-mail: peracoli@ibb.unesp.br

Abstract

Citation Peraçoli MTS, Bannwart CF, Cristofalo R, Medeiros Borges VT, Araújo Costa RA, Witkin SS, Peraçoli JC. Increased reactive oxygen species and tumor necrosis factor-alpha production by monocytes are associated with elevated levels of uric acid in pre-eclamptic women. Am J Reprod Immunol 2011; 66: 460–467

Problem  To evaluate associations between hyperuricemia and increases in production of reactive oxygen species (ROS) and tumor necrosis factor alpha (TNF-α) in pre-eclamptic pregnancies.

Method of study  This study investigated serum uric acid levels, monocyte production of TNF-α, superoxide anion (O2) and hydrogen peroxide (H2O2), as well as superoxide dismutase (SOD) and catalase (CAT) activities in erythrocytes from 30 women with pre-eclampsia (PE) compared with 30 normotensive (NT) pregnant women in the last trimester of pregnancy.

Results  Serum uric acid levels (6.1 versus 2.8 mg/dL) as well as endogenous O2 (2.2 versus 1.6 nm), H2O2 (1.8 versus 1.4 nm) and TNF-α (91.6 versus 40.4 pg/mL) released from monocytes were significantly higher in the pre-eclamptic group than in the NT group (P < 0.05). SOD activity in erythrocytes was also significantly elevated in the PE group (5969.2 versus 4834.7 U/g Hb). No significant difference between groups was observed in relation to CAT activity.

Conclusions  Elevated serum uric acid levels are correlated with higher O2 and TNF-α production by monocytes in women with PE. This may contribute to the enhanced oxidative and inflammatory state characteristic of this disorder.

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