Influence of Mid-Trimester Amniotic Fluid on Endogenous and Lipopolysaccharide-Mediated Responses of Mononuclear Lymphoid Cells

Authors

  • Steven S. Witkin,

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
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  • Joseph Chervenak,

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
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  • Ann Marie Bongiovanni,

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
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  • Catherine Herway,

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
    2. Department of Obstetrics and Gynecology, New York Hospital Queens Medical Center, New York, NY, USA
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  • Iara M. Linhares,

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
    2. Department of Gynecology and Obstetrics-Hospital das Clinicas, University of Sao Paulo Medical School, Sao Paulo, Brazil
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  • Daniel Skupski

    1. Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
    2. Department of Obstetrics and Gynecology, New York Hospital Queens Medical Center, New York, NY, USA
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Steven S. Witkin, Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, 525 East 68th Street, Box 35, New York, NY 10065, USA.
E-mail: switkin@med.cornell.edu

Abstract

Citation Witkin SS, Chervenak J, Bongiovanni AM, Herway C, Linhares IM, Skupski D. Influence of mid-trimester amniotic fluid on endogenous and lipopolysaccharide-mediated responses of mononuclear lymphoid cells. Am J Reprod Immunol 2012; 67: 28–33

Problem  We evaluated the influence of amniotic fluid (AF) on immune mediator production by mononuclear leukocytes.

Method of study  Thirty mid-gestation AFs were incubated with peripheral blood mononuclear cells (PBMCs) in the presence or absence of lipopolysaccharide (LPS). Supernatants were tested for interleukin (IL) – 6, 10, 12, 23, tumor necrosis factor-α (TNF-α) and monocyte chemotactic protein (MCP)-1.

Results  Endogenous mediator production was minimal or non-detectable. AF stimulated endogenous MCP-1, IL-6 and TNF-α release. In the presence of LPS, production of MCP-1 and IL-10 by PBMCs was enhanced eight- to ninefold by AF. Release of IL-6 and IL-23 was enhanced less than twofold by the addition of AF while TNF-α production was unchanged. AF-stimulated mediator production was similar irrespective of pregnancy outcome.

Conclusion  Selective AF stimulation of LPS-mediated MCP-1 and IL-10 release may be a mechanism to promote antibody production and the influx of phagocytic cells to engulf pathogens while downregulating the production of pro-inflammatory cytokines.

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