Reduction in miR-141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell Line JEG-3
Article first published online: 4 JUL 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Special Issue: Marcus Wallenberg International Symposium in Comparative Reproductive Immunology, “Immunology at the fetal maternal interface: Basic science and clinical applications”, July 7–8th, 2011, Linköping University, Sweden
Volume 66, Issue Supplement s1, pages 57–62, July 2011
How to Cite
Morales-Prieto, D. M., Schleussner, E. and Markert, U. R. (2011), Reduction in miR-141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell Line JEG-3. American Journal of Reproductive Immunology, 66: 57–62. doi: 10.1111/j.1600-0897.2011.01037.x
- Issue published online: 4 JUL 2011
- Article first published online: 4 JUL 2011
- Submitted April 28, 2011; accepted May 10, 2011.
- post-transcriptional gene silencing;
Citation Morales-Prieto DM, Schleussner E, Markert UR. Reduction in miR-141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell line JEG-3. Am J Reprod Immunol 2011; 66 (Suppl. 1): 57–62
Starting from the peri-implantation period, leukemia inhibitory factor (LIF) is a major regulator of trophoblast functions. Micro-RNAs (miRNA) are short non-coding RNA sequences, which regulate expression of genes at post-transcriptional level. The influence of LIF on miRNA expression in trophoblastic cells has not yet been analyzed and was focus of this investigation.
Method of study
JEG-3 choriocarcinoma cells have been stimulated with LIF for 1, 2, 4, 6, and 24 hr. The expression of miR-9, miR-141, miR-21, miR-93, and let-7g has been analyzed by real-time PCR. Subsequently, miR-141 has been silenced and over-expressed to test its role in the proliferation of JEG-3 cells after 24 and 48 hr.
MiR-141 has been significantly downregulated by more than 50% after LIF stimulation, while miR-21 and miR-93 expression has been significantly upregulated. Silencing of miR-141 completely inhibited the proliferation of JEG-3 cells, while over-expression had no effect.
LIF regulates expression of miRNA in trophoblastic cells, which may be responsible for several functional effects induced by LIF.