Dr. Tanya Dimova held a post doc position at the 1Department of Clinical Microbiology/Clinical Immunology.
Maternal Foxp3 Expressing CD4+ CD25+ and CD4+ CD25− Regulatory T-Cell Populations are Enriched in Human Early Normal Pregnancy Decidua: A Phenotypic Study of Paired Decidual and Peripheral Blood Samples
Article first published online: 4 JUL 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Special Issue: Marcus Wallenberg International Symposium in Comparative Reproductive Immunology, “Immunology at the fetal maternal interface: Basic science and clinical applications”, July 7–8th, 2011, Linköping University, Sweden
Volume 66, Issue Supplement s1, pages 44–56, July 2011
How to Cite
Dimova, T., Nagaeva, O., Stenqvist, A.-C., Hedlund, M., Kjellberg, L., Strand, M., Dehlin, E. and Mincheva-Nilsson, L. (2011), Maternal Foxp3 Expressing CD4+ CD25+ and CD4+ CD25− Regulatory T-Cell Populations are Enriched in Human Early Normal Pregnancy Decidua: A Phenotypic Study of Paired Decidual and Peripheral Blood Samples. American Journal of Reproductive Immunology, 66: 44–56. doi: 10.1111/j.1600-0897.2011.01046.x
- Issue published online: 4 JUL 2011
- Article first published online: 4 JUL 2011
- Submitted April 19, 2011; accepted May 17, 2011.
- CD4+ CD25+ Foxp3+ cells;
- CD4+ CD25++ Foxp3+ cells;
- CD4+ CD25− Foxp3+ cells;
- human decidua;
- Treg cells
Citation Dimova T, Nagaeva O, Stenqvist A-Christin, Hedlund M, Kjellberg L, Strand M, Dehlin E, Mincheva-Nilsson L. Maternal Foxp3 Expressing CD4+ CD25+ and CD4+ CD25− Regulatory T-Cell Populations are Enriched in Human Early Normal Pregnancy Decidua: A Phenotypic Study of Paired Decidual and Peripheral Blood Samples. Am J Reprod Immunol 2011; 66 (Suppl. 1): 44–56
Problem Regulatory T cells (Treg cells), a small subset of CD4+ T cells maintaining tolerance by immunosuppression, are proposed contributors to the survival of the fetal semiallograft. We investigated Treg cells in paired decidual and peripheral blood (PB) samples from healthy women in early pregnancy and PB samples from non-pregnant women.
Method of study Distribution, location, cytokine mRNA, and phenotype were assessed in CD4+ CD25+ Treg cells from paired samples using immunohistochemistry, immunofluorescence, flow cytometry, and real-time quantitative RT-PCR.
Results The presence and in situ distribution of CD4+ Foxp3+ Treg cells in decidua are hereby demonstrated for the first time. Three Foxp3+ cell populations, CD4+ CD25++ Foxp3+, CD4+ CD25+ Foxp3+, and CD4+ CD25− Foxp3+, were enriched locally in decidua. In contrast, no statistically significant difference in numbers of circulating Treg cells between pregnant and non-pregnant women was found. The Foxp3+ cells expressed the surface molecules CD45RO, CTLA-4, CD103, Neuropilin-1, LAG-3, CD62L, and TGFβ1 mRNA consistent with Treg phenotype. The population of CD4+ CD25− Foxp3+ cells, not described in human decidua before, was enriched 10-fold compared with PB in paired samples. Their cytokine expression was often similar to Th3 profile, and the Foxp3 mRNA expression level in CD4+ CD25− cells was stable and comparable to that of CD4+ CD25+ Treg cells implying that the majority of CD4+ CD25− Foxp3+ cells might be naïve Treg cells.
Conclusion (i) There is a local enrichment of Treg cells in decidua (ii) The exclusive accumulation of decidual CD4+ CD25− Foxp3+ cells suggests an additional reservoir of Foxp3+ naïve Treg cells that can be converted to ‘classical’ Treg cells in uterus.