In Vitro Secretion Profile of Pro-Inflammatory Cytokines IL-1β, TNF-α, IL-6, and of Human Beta-Defensins (HBD)-1, HBD-2, and HBD-3 from Human Chorioamniotic Membranes After Selective Stimulation with Gardnerella vaginalis
Article first published online: 13 JUL 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 1, pages 34–43, January 2012
How to Cite
Zaga-Clavellina, V., Martha, R. V.-M. and Flores-Espinosa, P. (2012), In Vitro Secretion Profile of Pro-Inflammatory Cytokines IL-1β, TNF-α, IL-6, and of Human Beta-Defensins (HBD)-1, HBD-2, and HBD-3 from Human Chorioamniotic Membranes After Selective Stimulation with Gardnerella vaginalis. American Journal of Reproductive Immunology, 67: 34–43. doi: 10.1111/j.1600-0897.2011.01054.x
- Issue published online: 12 DEC 2011
- Article first published online: 13 JUL 2011
- Submitted April 19, 2011; accepted June 2, 2011.
- Antimicrobial peptides;
- chorioamniotic membranes;
- human beta defensins;
- intrauterine infection;
- pro-inflammatory cytokines
Citation Zaga-Clavellina V, Martha RV-M, Flores-Espinosa P. In vitro secretion profile of pro-inflammatory cytokines IL-1β, TNF-α, IL-6, and of human beta-defensins (HBD)-1, HBD-2, and HBD-3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immunol 2012; 67: 34–43
Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis.
Method of study
Using a two-compartment experimental model, 1 × 106 CFU/mL of G. vaginalis were added to either the amnion or choriodecidua face or to both. Concentrations of IL-1β, TNF-α, and IL-6, as well as human beta defensins (HBD) 1-3 were quantified by ELISA.
In comparison with control conditions and regardless of the stimulation modality, IL-1β and IL-6 increased 4-fold and 28-fold, respectively, in the choriodecidual compartment. HBD-1 increased 2-fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD-2 and HBD-3 increased an average of 2- and 8-fold, respectively, in the choriodecidual region.
Stimulation with G. vaginalis induced a tissue-specific secretion profile of 1L-1β, IL-6, and HBD 1-3 in the chorioamniotic membranes.