Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells
Article first published online: 28 AUG 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 1, pages 73–83, January 2012
How to Cite
Borbely, A. U., Fontenele-Neto, J. D., Vidsiunas, A. K., Gomes, S. Z., Hoshida, M. S., de Oliveira, S. F. and Bevilacqua, E. (2012), Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells. American Journal of Reproductive Immunology, 67: 73–83. doi: 10.1111/j.1600-0897.2011.01060.x
- Issue published online: 12 DEC 2011
- Article first published online: 28 AUG 2011
- Submitted April 18, 2011; accepted July 12, 2011.
- Cell death;
- coculture assay;
- mesometrial decidual cells;
- TUNEL reaction
Citation Borbely AU, Fontenele-Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)-γ-treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83
Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)-γ, in the presence or absence of ectoplacental cone (EC) using a coculture system.
Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN-γ, and cell death was evaluated.
Results Interferon-γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN-γ-treated decidual culture. Moreover, the addition of EC to non-treated DC cultures was also capable of attenuating death rates.
Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN-γ-dependent apoptosis and, therefore, play important role for DC survival in a cytokine-enriched placental environment.