Vascular Endothelial Growth Factor Gene Polymorphisms in Spontaneously Aborted Fetuses


Nam Keun Kim, PhD, Institute for Clinical Research, CHA Bundang Medical Center, CHA University, 351, Yatap-Dong, Bundang-gu, Seongnam 463-712, South Korea. E-mail:
Sung Han Shim, PhD, Fertility Center of CHA Gangnam Medical Center, CHA University, 606-13, Yeoksam-dong, Gangnam-gu, Seoul 135-081, South Korea.


Citation Jeon YJ, Kim JH, Rah HC, Kim SY, Yoon TK, Choi DH, Cha SH, Shim SH, Kim NK. Vascular endothelial growth factor gene polymorphisms in spontaneously aborted fetuses. Am J Reprod Immunol 2011; 66: 544–553

Problems  The VEGF−1154G>A polymorphism has been reported to be a genetic risk factor for recurrent spontaneous abortion in various studies; however, these studies have focused on genetic analyses of pregnant women rather than aborted fetuses. To evaluate and confirm the association between the VEGF−1154G>A polymorphism and spontaneous abortion, we focused on the relationship between four polymorphisms in the VEGF gene (−2578C>A, −1154G>A, −634G>C, and 936C>T) and spontaneously aborted fetuses (SAFs).

Method of study  The subjects included 118 SAFs at <20 weeks gestation and 380 normal controls consisting of children and adults. The polymorphisms were genotyped by polymerase chain reaction–restriction fragment length polymorphism analysis.

Results  Spontaneously aborted fetuses exhibited significantly different frequencies of the −2578CA+AA/−634CC and −1154GA+AA/−634CC combined genotypes compared with control subjects. The frequency of the −2578A/−1154A/−634C/936C haplotype was significantly higher in SAFs.

Conclusions VEGF genes −2578CA+AA/−634CC and −1154GA+AA/−634CC in the fetus are possible risk factors for spontaneous abortion.